基于蛋白芯片技术探讨丁氏溃结灌肠液对结肠炎大鼠肠道组织差异蛋白表达的影响  

Effect of Ding's herb enema prescription on intestinal tissue related differences target in rat colitis using protein array technology

作　　者：丁康[1] 张辉[2] 谭妍妍[1] 朱维娜[3] 李芷薇 陆蔚 李睿瑛 赵敏[5] 丁洋 李猛 黄士财 丁义江[1] 张苏闽[1] 

机构地区：[1]南京中医药大学第三附属医院肛肠科,江苏南京210001 [2]南京中医药大学第三附属医院检验科,江苏南京210001 [3]南京中医药大学第三附属医院中心实验室,江苏南京210001 [4]南京中医药大学药学院,江苏南京210023 [5]南京中医药大学第一临床医学院,江苏南京210023

出　　处：《中国药理学通报》2017年第10期1473-1478,共6页Chinese Pharmacological Bulletin

基　　金：江苏省自然科学基金资助项目(No BK-20151082);南京市卫计委重点项目(No ZKX16056);江苏省普通高校专业学位研究生实践创新计划项目(No SJZZ16_0174);2016年度博士后科研资助项目(No BSH201501)

摘　　要：目的基于蛋白芯片技术探讨丁氏溃结灌肠液对结肠炎大鼠肠道组织相关靶点的影响,阐释丁氏溃结灌肠液改善肠道炎症反应及肠纤维化的可能机制。方法大鼠随机分为阳性药组、模型组、正常组、丁氏溃结灌肠液组,5只/组。除正常组之外,其他3组均给予3.5%硫酸葡聚糖钠(dextran sulfate sodium,DSS)喂养,结肠炎造模成功后,阳性药组使用美沙拉秦灌肠,丁氏溃结灌肠液组使用丁氏溃结灌肠液灌肠,每天1次,连续4周,停药1 d后,取大鼠结肠黏膜组织,采用GSR-CAA-67抗体蛋白芯片技术检测各组大鼠肠道组织炎症反应及肠纤维化相关蛋白的变化,筛选出各组差异性表达的蛋白。结果通过蛋白芯片检测,发现共有8种有意义的差异蛋白,其中模型组和空白组对照组对比筛选出的8种蛋白包括IFN-γ、促红细胞生成素(erythropoietin,EPO)、TIMP-2、TIM-1、IL-6、TIMP-1、TNF-α、IL-22,差异均有统计学意义(P<0.05)。另一方面,丁氏溃结灌肠液与美沙拉秦均对IL-6、TIMP-1有明显的抑制作用(P<0.05),丁氏溃结灌肠液对TNF-α、IL-22有抑制作用(P<0.05),美沙拉秦组则没有相同作用(P>0.05)。结论丁氏溃结灌肠液具有多靶点的作用,可能通过改善肠道炎症反应及肠纤维化达到治疗溃疡性结肠炎的目的。Aim To investigate the effect of Ding ＇s herb enema prescription on intestinal tissue related target in rat colitis induced by dextran sulfate sodium（ DSS）,and to elucidate the mechanism of Ding＇s herb enema prescription in improving the intestinal inflammation and intestinal fibrosis. Methods Rats were fed with 3. 5% DSS. The rats were randomly divided into positive drug group,model group,Control group,and Ding ＇s herb enema prescription group.The positive drug group was treated with mesalazine enema,and Ding＇s herb enema prescription group was treated with Ding＇s herb enema prescription. The colon mucosa was taken once a day for 6 weeks. The changes of intestinal inflammatory response and intestinal fibrosis related proteins were detected by GSRCAA-67 antibody protein array,and the differentially expressed proteins were screened out. Results Eight proteins showed statistical differences,including IFN-γ, erythropoietin（ EPO）, TIMP-2, TIM-1, IL-6,TIMP-1,TNF-α,IL-22（ P 〈 0. 05）. On the other hand,Ding＇s herb enema prescription and mesalazine significantly antagonized the effect of IL-6 and TIMP-1（ P 〈 0. 05）. The antagonized effect of Ding＇s embolization enema on TNF-α and IL-22 was also significant（ P 〈 0. 05）,but mesalazine had no similar effect（ P 〉 0. 05）. Conclusions Ding＇s herb enema prescription has the effect of multiple targets,which may improve the intestinal inflammatory response and intestinal fibrosis to achieve the purpose of treatment of ulcerative colitis（ UC）.

关 键 词：蛋白芯片 丁氏溃结灌肠液 差异蛋白 溃疡性结肠炎 大鼠 肠纤维化 

分 类 号：R-332[医药卫生] R289.1