微小RNA-7敲减对ConA诱导的小鼠急性肝损伤的影响  

Effect of microRNA-7 knockdown on Con A-induced acute liver injury in mice

作　　者：岳东旭[1,2] 赵娟娟[1,2] 张忆雄[1,2] 丁涛[1,2] 陈慧子 徐林[1,2] 

机构地区：[1]遵义医学院免疫学教研室暨贵州省生物治疗人才基地,贵州遵义563099 [2]遵义医学院贵州省普通高等学校特色药物肿瘤防治特色重点实验室,贵州遵义563099

出　　处：《中国病理生理杂志》2017年第9期1643-1647,共5页Chinese Journal of Pathophysiology

基　　金：国家自然科学基金资助项目(No.31370918);贵州省高层次创新人才计划(黔科合人才(2016)4031号);遵义医学院优秀青年人才计划项目(No.15ZY-001)

摘　　要：目的:研究微小RNA-7(miR-7)敲减(KD)对急性肝损伤(ALI)模型小鼠的影响。方法:野生型(WT)小鼠和miR-7KD小鼠腹腔注射30 mg/kg刀豆蛋白(ConA)建立急性肝损伤模型;48 h后,观察小鼠肝脏的形态、重量及其脏器指数变化;HE染色观察小鼠肝脏组织病理学变化;血清学方法检查血清中谷丙转氨酶(ALT)的水平;ELISA法检测血清中细胞因子IL-4和IFN-γ的水平;流式细胞术检测肝脏组织中CD4^+T细胞的比例及其相关的细胞因子IL-4和IFN-γ的表达变化。结果:与对照组相比,miR-7敲减后急性肝损伤小鼠的肝脏组织颜色变浅,重量减轻,重量指数明显增加(P<0.05);HE染色显示miR-7KD小鼠血清炎症细胞浸润显著增多;血清学方法检测发现急性肝损伤小鼠血清ALT的水平明显上升(P<0.05);ELISA法检测显示miR-7KD小鼠血清中IFN-γ水平明显升高(P<0.01),而IL-4的表达水平则明显降低(P<0.01);流式细胞术检测结果显示,miR-7KD小鼠肝脏中CD4+T细胞比例显著升高(P<0.01),其相关的细胞因子IFN-γ的表达水平也显著上调(P<0.01),而IL-4的水平没有发生明显变化。结论:敲减miR-7基因可明显促进ConA诱导的小鼠急性肝损伤。AIM： To study the effect of microRNA-7（miR-7） knockdown（KD） on concanavalin A（Con A）-induced acute liver injury（ ALI） in mice. METHODS： Wild type（ WT） mice and miR-7KD mice were received Con A（30 mg/kg） to induced acute liver injury model by intraperitoneal injection,and the morphological changes,liver weight and weight index were measured 48 h later. The pathological changes of the liver tissues were observed by HE staining. The levels of serum alanine aminotransferase（ ALT）,IL-4 and IFN-γ were detected by ELISA. The proportional changes of CD4~＋T cells and the relative levels of IL-4 and IFN-γ were analyzed by flow cytometry. RESULTS： The color of the liver tissue became lighter,and the weight and weight index were changed significantly in miR-7KD mice compared with control group（ P 0. 05）. HE staining showed that the inflammatory cell infiltration was increased in the liver of miR-7KD mice.Moreover,the level of serum ALT was significantly increased（ P 0. 05）. The serum level of IFN-γ elevated significantly（P 0. 01）,while the IL-4 levels decreased significantly（P 0. 01） in the serum of miR-7KD mice. Furthermore,the proportion of CD4~＋T cells and relative IFN-γ cells increased obviously（ P 0. 01）. CONCLUSION： miR-7 knockdown promotes the pathogenesis of the Con A-induced acute liver injury in mice.

关 键 词：微小RNA-7 急性肝损伤 CD4^+T细胞 

分 类 号：R363[医药卫生—病理学]