新加大黄附子汤对小鼠原位移植肝癌的治疗作用  

The therapeutic effect of the new added Dahuangfuzi Decoction on orthotopic liver cancer in mice

作　　者：李自波 姚静静 杜振华 杜钢军[1] 

机构地区：[1]河南大学药学院药物研究所,河南开封475004

出　　处：《河南大学学报（医学版）》2017年第3期156-159,共4页Journal of Henan University:Medical Science

基　　金：国家自然科学基金项目(81173094)

摘　　要：[目的]观察新加大黄附子汤对小鼠原位移植肝癌是否具有治疗效果。[方法]建立小鼠原位移植肝癌模型,造模小鼠随机分组,次日用新加大黄附子汤灌胃开始治疗,观察小鼠的生存状态,用药40 d后测量小鼠肝脏的质量,计算肿瘤的体积,检测血清CEA肿瘤标志物,HE染色检测小鼠肝脏病理变化。[结果]模型组小鼠原位移植肝癌的致瘤率为95%,小鼠自主活动、体温都偏低,40 d存活率只有45%,肿瘤体积较大;新加大黄附子汤治疗的小鼠致瘤率为75%,小鼠的自主活动、体温都高于模型组,40 d存活率达到70%,肝脏肿瘤体积明显小于模型组(P<0.05);CEA肿瘤标志物检测发现给药组含量明显低于模型组(P<0.05)。[结论]新加大黄附子汤对小鼠原位移植肝癌具有治疗作用。[Objective]To observe therapeutic effect of the new added Dahuangfuzi Decoction on orthotopic liver cancer in mice. [MetliO（is]The mice were divided into different groups randomly after orthotopic liver cancer model in mice was established.The mice were received the new added Dahuangfuzi Decoction via intragastric administration at the second day following tumor inoculation. The survival status of mice was observed every day. The tumor volume and liver weight were measured after 40 days. The concentration of CEA in the serum was detected and the liver pathological changes were observed by HE staining. [Results] The tumorigenicity rate in the model group was 95%.The autonomic activities and the temperature of the model group were lower than the therapeutic group, and the survival rate was only 45%. The tumorigenicity rate in the therapeutic group was 75% and the survival rate was 70%. Compared with the model group, the concentration of CEA and tumor volume in the therapeutic group were obviously lower （P 〈 0.05j. [Conclusion ]The new added Dahuangfuzi Decoction have therapeutic effect on orthotopic liver cancer in mice.

关 键 词：新加大黄附子汤 肝癌 原位移植 治疗作用 

分 类 号：R735.7[医药卫生—肿瘤]