长链非编码RNA-Linc00152与肝细胞癌的临床病理特征关系  

Correlation between long non-coding RNA-Linc00152 and clinicopathological feature of hepatocellular carcinoma

作　　者：刘杰[1] 华学锋 吕嘉贤 符荣党 李宁[2] 陈湖岸[1] 邱春辉[1] 赵辉[1] 陆敏强[1] 

机构地区：[1]中山大学附属第三医院肝脏外科,广州510630 [2]广州市第一人民医院,广州510180 [3]中山大学附属第一医院,广州510080 [4]佛山市第一人民医院,佛山528000

出　　处：《新医学》2017年第9期624-628,共5页Journal of New Medicine

基　　金：国家自然科学基金(81170450;81400679);广东省基础与应用基础研究专项基金(2014A030310067)

摘　　要：目的筛选出在肝细胞癌组织中差异表达的长链非编码RNA(LncRNA),研究其表达水平与肝细胞癌临床病理特征的相关性。方法收集40对肝细胞癌与配对的正常肝组织标本,使用基因芯片结合权威数据库筛选出在肝细胞癌组织中差异表达的LncRNA,预试验确定作为研究目标的LncRNA,使用实时荧光定量PCR研究目标LncRNA在肝细胞癌组织中的表达情况,并分析其表达与肝细胞癌临床病理特征的相关性。结果选取基因芯片中差异表达倍数最明显的10个候选LncRNA进行预试验,确定Linc00152作为研究目标。85%(34/40)的肝细胞癌组织中Linc00152相对表达量高于配对正常肝组织。肝细胞癌低分化者的Linc00152低表达者比例高于中分化者(P<0.017),有血管侵犯者的Linc00152低表达者比例高于无血管侵犯者(P<0.05)。结论 LncRNA-Linc00152在肝细胞癌组织中表达升高,且和肝细胞癌的病理分级和血管侵犯相关。Objective To screen the long non-coding RNA （LncRNA） differentially expressed in the hepatocellular carcinoma tissues and analyze the correlation between the expression level of LncRNA and clinicopathological feature of hepatocellular carcinoma.Methods Forty pairs of hepatocellular carcinoma and matched liver normal tissue specimens were collected.Gene microarray combined with official databases to screen the LncRNA differentially expressed in hepatocellular carcinoma tissues.In preliminary experiment, the target LncRNA was determined.Fluorescent quantitative RT-PCR was adopted to quantitatively measure the expression level of target LncRNA in hepatocellular carcinoma tissues and analyze the correlation between the expression level of LncRNA and clinicopathological characteristics of hepatocellular carcinoma.Results Ten candidate LncRNAs which were the most differentially expressed detected by gene microarray were chosen for preliminary experiment.Linc00152 was determined as the target LncRNA.The relative expression levels of Linc00152 in 85% （34/40） of hepatocellular carcinoma tissues were up-regulated compared with those in matched liver normal tissues. The percentage of low expression level of Linc00152 in poorly-differentiated hepatocellular carcinoma tissues was significantly higher than that in middle-differentiated counterparts（P＆lt;0.017）. The proportion of low expression level of Linc00152 in tissues with vascular invasion was considerably higher compared with that in those without vascular invasion （P＆lt;0.05）. Conclusions The expression level of LncRNA-Linc00152 is up-regulated in hepatocellular carcinoma tissues. It is associated with the pathological grading of hepatocellular carcinoma and vascular invasion.

关 键 词：长链非编码核糖核酸 肝细胞癌 Linc00152 

分 类 号：R735.7[医药卫生—肿瘤]