前列腺癌病灶内GPRC6A、PKCzeta表达与细胞增殖、EMT的关系研究  

Relations of GPRC6A and PKCzeta expression levels in prostate cancer lesions with cell proliferation and EMT

作　　者：曾繁飞[1] 田生平[1] 杨伟忠[1] 张小悦[1] 

机构地区：[1]广东省惠州市第三人民医院泌尿外科,广东惠州516000

出　　处：《海南医学院学报》2017年第15期2153-2156,共4页Journal of Hainan Medical University

基　　金：惠州市科技计划项目(2016Y154)

摘　　要：目的:研究前列腺癌病灶内G蛋白偶联受体C6A(G protein-coupled receptor class C group 6member A,GPRC6A)和蛋白激酶Cζ(Protein Kinase Czeta,PKCzeta)表达水平与细胞增殖、上皮间质转化(EMT)的关系。方法:选择2014年6月~2017年3月期间在我院接受手术切除治疗的前列腺癌患者以及良性前列腺增生患者,留取前列腺癌患者的前列腺癌病灶、癌旁病灶适量,检测病灶中GPRC6A、PKCzeta、细胞增殖基因、EMT基因的表达量。结果:前列腺癌病灶、癌旁病灶中GPRC6A、Survivin、SRSF1、Bcl-xl、N-cadherin、Vimentin的表达量显著高于良性前列腺增生病灶,PKCzeta、Caspase-3、Caspase-9、Apaf-1、E-cadherin、CK5/6的表达量显著低于良性前列腺增生病灶;PKCzeta低表达的前列腺癌病灶中Survivin、SRSF1、Bcl-xl的表达量显著高于PKCzeta高表达的前列腺癌病灶,Caspase-3、Caspase-9、Apaf-1的表达量显著低于PKCzeta高表达的前列腺癌病灶;GPRC6A低表达的前列腺癌病灶中E-cadherin、CK5/6的表达量显著高于GPRC6A高表达的前列腺癌病灶,N-cadherin、Vimentin的表达量显著低于GPRC6A高表达的前列腺癌病灶。结论:前列腺癌病灶内高表达的GPRC6A和低表达的PKCzeta分别能够促进细胞EMT和增殖。Objective： To study the relations of GPRC6A and PKCzeta expression levels in prostate cancer lesions with cell proliferation and epithelial-mesenehymal transition （EMT）. Methods： Patients with prostate cancer and patients with benign prostatic hyperplasia who received surgical resection in the Third People＇s Hospital of Huizhou City between June 2014 and March 2017 were selected as the research subjects. Prostate cancer lesion and the lesion adjacent to carcinoma were properly collected from patients with prostate cancer, and then the expression of GPRC6A, PKCzeta, cell proliferation genes and EMT genes in these lesions were detected. Results： GPRC6A, Survivin, SRSF1, Bcl-xl, N cadherin and Vimentin expression in prostate cancer lesions and adjacent lesions were significantly higher than those in benign prostatic hyperplasia lesions （P〈0.05）while PKCzeta, Caspase-3, Caspase-9, Apaf-1, E-cadherin and CK5/6 expression were significantly lower than those in benign prostatic hyperplasia lesions （P ~0.05） ; Survivin, SRSF1 and Bcl-xl expression in prostate cancer lesions with lower PKCzeta expression were significantly higher than those in prostate cancer lesions with higher PKCzeta expression （P〈0.05） while Caspase-3, Caspase-9 and Apaf-1 expression were significantly lower than those in prostate cancer lesions with higher PKCzeta expression （P〈0.05）; E-cadherin and CK5/6 expression in prostate cancer lesions with lower GPRC6A expression were significantly higher than those in prostate cancer lesions with higher GPRC6A expression （P〈0.05） while N-cadherin and Vimentin expression were significantly lower than those in prostate cancer lesions with higher GPRC6A expression （P 〈0.05）. Conclusions.. Highly expressed GPRC6A and lowly expressed PKCzeta in prostate cancer lesions can promote cell EMT and proliferation respectively.

关 键 词：前列腺癌 G蛋白偶联受体C6A 蛋白激酶Cζ 增殖 上皮间质转化 

分 类 号：R737.25[医药卫生—肿瘤]