Apelin和血管紧张素受体AT1相关受体蛋白在胃癌中表达及其临床意义  

The expression of apelin and putative receptor protein related to the angiotensin receptor AT1 in human gastric cancer and their clinicopathologic significance

作　　者：张慧 李建军 李雷[3] 赵丽华[3] 王爱亮[4] 冯其贞 Zhang Hui Li Jianjun Li Lei Zhao Lihua Wang A iliang Feng Qizhen(Department of Histology and Embryology Clinical College 5.School of Rehabilitation Medicine, lining Medical University, Jining 272067, Chin 3Department of Patholog 4.Department of Gastroenterological Surgery ,Affiliated Hospital of Jining Medical University, Jining 272029, Chin)

机构地区：[1]济宁医学院基础医学院组织学与胚胎学教研室,5272067 [2]济宁医学院临床医学院,5272067 [3]济宁医学院附属医院病理科,4272029 [4]济宁医学院附属医院胃肠外科,4272029 [5]济宁医学院康复医学院,5272067

出　　处：《中华诊断学电子杂志》2017年第3期196-199,共4页Chinese Journal of Diagnostics(Electronic Edition)

基　　金：山东省自然科学基金(ZR2013HL011)

摘　　要：目的探讨胃癌组织中apelin和血管紧张素受体AT1相关受体蛋白(APJ)的表达及其临床意义。方法标本来源于2015年1月至12月济宁医学院附属医院胃肠外科收治的123例胃癌患者,自医院病理科调取上述患者的癌组织及正常黏膜组织的蜡块,采用SP免疫组织化学染色法对标本中apelin、APJ的表达水平并进行了半定量分析,通过计算CD34标记的微血管评价"热点"血管密度(hMVD)。结果 Apelin和APJ在正常胃黏膜的腺细胞均呈阳性表达、但是在上皮层未见有表达;apelin主要表达于胃癌细胞的细胞质和细胞核,但未见APJ在胃癌细胞中的表达;CD34在肿瘤组织中微血管计数明显高于正常胃粘膜;Apelin的表达水平在不同的胃癌患者年龄、性别和TNM分期之间的差异无统计学意义,肿瘤的分化程度(3.16±2.10,3.55±2.01,4.36±2.18;F=20.56,P<0.05)和微"热点"血管密度(3.35±2.14,4.23±2.05;z=12.37,P<0.05)差异有统计学意义。结论Apelin蛋白参与了胃癌的发生发展,其表达水平与肿瘤分化程度、hMVD有关,在低分化、高hMVD的肿瘤组织中呈现较高表达水平。Objective To study the expression level of apelin and angiotensin receptor AT1 （APJ） in gastric cancer and explore possible association between the expression of Apelin/APJ and clinical significance.Methods Cancer tissues and normal mucosa tissues with wax block of 123 cases of gastric cancer patients, who received surgery of gastrointestinal surgery in Jining medical college affiliated hospital from January 2015 to December 2015 were collected from pathology department. SP immunohistochemical staining was used to explore the expression level of apelin and APJ. Semi-quantitative analysis was carried out.Through calculating microvasculars labelled by CD34 markers,＂ hot spots＂ microvessel densitic （hMVD） was calculated. Results Both apelin and APJ showed positive expression in cytoplasm of normal gastric mucosa,but no expression was found in the upper cortex.Apelin was mainly expressed in the cytoplasm and nucleus of gastric cancer cells, but no APJ was expressed in gastric cancer. CD34 was significantly higher in tumor tissue than that in normal gastric mucosa. There were no significant difference for apelin expressing levels in different age （ 3.56±2.13,3.98±2.08 ）, sex （3.62±2.21,3.96±2.27） and TNM （ 3.66±2.36,4.14±2.12） staging of gastric cancer patients. However, there were significant differences between different tumor differentiation degree （ 3.16±2.10, 3.55±2.01,4.36±2.18; F = 20.56, P 〈0.05 ） and high-microvessel densitie （hMVD） （3.35±2.14,4.23±2.05; z = 12.37, P 〈0.05）.Conclusions Apelin is involved in the development of gastric cancer, and its expression level is related to the degree of tumor differentiation and hMVD.High expression level of apelin is presented in tumor tissues with low differentiation and high density hMVD.

关 键 词：APELIN 血管紧张素受体AT1相关的受体蛋白 抗原 CD34 胃肿瘤 

分 类 号：R735.2[医药卫生—肿瘤]