抗菌治疗对兔颊VX-2鳞癌感染性微环境中树突状细胞的影响  

The effects of antimicrobial therapy on dendritic cells in the infected microenvironment of rabbit buccal VX-2 squamous cell carcinoma

作　　者：陈志红[1] 易杰[2] 黄桂林[2] 张霓霓[2] 姚礼[2] 张立刚[2] 

机构地区：[1]贵州省人民医院口腔科,贵阳550002 [2]遵义医学院附属口腔医院口腔颌面外科

出　　处：《实用口腔医学杂志》2017年第5期594-597,共4页Journal of Practical Stomatology

基　　金：贵州省科学技术基金(编号:黔科合J字LKZ[2013]36号;黔科合J字LKZ[2011]23号);遵义市红花岗区科学技术局项目(编号:遵红科合社字[2015]09号);贵州省研究生工作站项目(编号:黔教研合YJSZ字[2013]08)

摘　　要：目的:探索抗菌治疗对兔颊VX-2鳞癌感染性微环境中树突状细胞(dendritic cells,DCs)成熟及功能的影响。方法:瘤粒植入形成兔颊VX-2鳞癌后,附加机械创伤及高糖饮食获得该鳞癌的炎症模型,再分为3组(n=6),A组:颊癌炎症模型使用抗生素治疗3 d;B组:颊癌炎症模型以生理盐水代替抗生素对照;C组:单纯颊癌不做处理。3 d后收集各组肿瘤标本,制成匀浆,取上清液与正常兔外周血单核细胞共培养,流式细胞仪检测DCs表面分子HLA-DR、CD83、CD86的表达,混合淋巴细胞反应检测DCs刺激T细胞增殖的能力。结果:HLA-DR、CD83、CD86阳性率及刺激指数(stimulate index,SI)由高到低均分别为:C组>A组>B组(P<0.05)。结论:抗菌治疗有助于感染兔颊VX-2鳞癌微环境中树突状细胞的成熟及功能恢复。Objective： To study the effects of antimicrobial therapy on the maturation and function of dendritic cells（DCs） in the infected microenvironment of rabbit buccal VX-2 squamous cell carcinoma. Methods： The inflammatory models were obtained by mechanical trauma and high sugar diet on the basis of rabbit buccal VX-2 squamous cell carcinoma models which were established by particle implantation of the tumor tissue. The model rabbits were divided into 3 groups （ n = 6）. In group A the rabbits with buccal VX-2 squamous cell carcinoma and local inflammation were given antibiotics by garage and intramuscular injection for 3 consecutive days; the rabbits in group B with buccal VX-2 squamous cell carcinoma and local inflammation were given normal saline by gavage and intramuscular injection; the rabbits in group C with tumor and without inflammation were given normal saline by gavage and intra- muscular injection. The tumor specimens were collected 3 days after treatment, and made into tissue homogenate, supernatant was col- lected after centrifugation. Normal rabbit peripheral blood mononuelear cells were separated and co-cultured with the supernatant ob- tained from the 3 groups respectively. Expressions of DCs surface markers HLA-DR,CD83 and CD86 were detected by flow cytometry. the function of DCs was tested by mixed lymphocyte reaction. Results： The positive rate of HLA-DR, CD83, CD86 and stimulate index were group C 〉 group A 〉 group B （ P 〈 0.05 ）. Conclusion： Antimicrobial therapy can promote the maturation and function of DCs in the infected microenvironment of rabbit buccal VX-2 squamous cell carcinoma.

关 键 词：抗菌治疗 炎症 VX-2鳞癌 树突状细胞 

分 类 号：R739.8[医药卫生—肿瘤]