七叶一枝花-壳聚糖混合物作为义齿稳固剂的生物安全性及生物相容性研究  

The biological safety and biocompatibility evaluation of the mixture of Paris polyphylla-chitosan for denture adhesive

作　　者：张雅琳 孟贺 李金源 吴文慧 梁锐英 

机构地区：[1]华北理工大学口腔医学院修复教研室,唐山063000

出　　处：《实用口腔医学杂志》2017年第5期617-620,共4页Journal of Practical Stomatology

摘　　要：目的:评价七叶一枝花-壳聚糖混合物的生物安全性及生物相容性。方法:参照国家标准GB/T 16886.12-2005、YY/T 0279-1995及GBT16886.5-2003规定的方法,将七叶一枝花-壳聚糖混合物按0.1 g/ml的标准放入浸提介质中,37℃条件下、浸提24 h制备浸提液,采用口腔黏膜刺激试验、细胞毒性试验以及流式细胞术初步评价七叶一枝花-壳聚糖混合物的生物安全性及生物相容性。结果:金黄地鼠口腔黏膜与试样接触部位未见出血、肿胀,组织学观察未出现病理性改变,七叶一枝花-壳聚糖混合物无口腔黏膜刺激性;对L929细胞无细胞毒性;对L929细胞的周期及凋亡无显著影响。结论:初步认为七叶一枝花-壳聚糖混合物具有良好的生物安全性和生物相容性。Objective： To study the biological safety and biocompatibility of the mixture of Paris polyphyllachitosan. Methods： According to the GB/T 16886.12 -2005 standard, YY/T 0279 -1995 standard and GBT16886.5 -2003 standard, samples were prepared and tested by oral mucous membrane irritation test, cytotoxicity test and flow cytometry. Results： No local response to the mixture of Paris polyphylla-chitosan was found, and the visual observation and pathological findings of oral mucosa were normal and similar to that of the control group. Therefore, the mixture of Paris polyphylla-chitosan had no irritation response to oral mucosa. The mixture of Paris polyphylla-chitosan showed no cytotoxicity to L929 cells,and did not affect the cycle distribution and apoptosis of L929 cells. Conclusion： The mixture of Paris polyphylla-chitosan has good bio-safety and biocompatibility.

关 键 词：七叶一枝花 壳聚糖 生物安全性 生物相容性 义齿稳固剂 

分 类 号：R783.1[医药卫生—口腔医学]