口腔鳞癌患者与健康人唾液差异蛋白组学的初步研究  

A primary study of the differential proteomic expression in saliva of health people and the patients with oral squamous cell carcinoma

作　　者：孙昆俊 马洪 康颖倩[2] 邹贤玉 

机构地区：[1]贵州医科大学口腔医学院口腔颌面外科学教研室,550004 [2]贵州医科大学蛋白组学重点实验室

出　　处：《实用口腔医学杂志》2017年第5期653-656,共4页Journal of Practical Stomatology

基　　金：2007年贵州省优秀人才省长基金[编号:黔省专合字(2006)116号]

摘　　要：目的:研究口腔鳞癌患者与健康人唾液蛋白差异表达情况。方法:收集口腔鳞癌患者唾液17例,健康人唾液17例,通过以双向凝胶电泳技术以及质谱鉴定和生物信息学分析,寻找在口腔鳞癌患者唾液中差异表达的蛋白质。结果:选取口腔鳞癌患者和正常人唾液中具有明显差异表达的10个蛋白质点,进行质谱鉴定和生物信息学分析,共鉴定出3种差异表达的蛋白质,其分别为S100A8、S100A8/S100A9及表皮角蛋白2(EK2)在口腔鳞癌患者唾液中均呈高表达。结论:S100A8、S100A8/S100A9及EK2可能与口腔鳞癌患者发生有关。Objective： To study the differentially expressed proteins in saliva of health people and the patients with oral squamous cell carcinoma（OSCC）. Methods： Saliva of 17 cases with OSCC and paired health subjects was collected, the proteins in the saliva were examined by two-dimensional gel electrophoresis （2-DE） separation, the proteins were examined by 2-DE separation, the saliva proteome dimensional electrophoresis profiles were obtained by MALDI-TOF/MS mass spectrometric identification, the information of the differentially expressed protein in OSCC group was studied by NCBI database bioinformatics analysis. Results： 10 proteins differentially expressed between the 2 groups were observed by mass spectrometry. Bioinformatics analysis showed that S100A8, S100A8/S100A9 and Epidermal cytokeratin 2（EK2） were highly expressed in the saliva of OSCC cases. Conclusion： S100A8; SI00A8/S10OA9 and EK2 may be related to the development of OSCC.

关 键 词：唾液 口腔鳞癌 蛋白质组学 双向电泳(2-DE) 肿瘤标志物 

分 类 号：R739.8[医药卫生—肿瘤]