人脐带Wharton’s Jelly来源间充质干细胞与人牙周膜干细胞成骨分化能力的对比研究  

The osteogenesis ability of human umbilical cord Wharton's Jelly-derived mesenchymal stem cells and periodontal mesenchymal stem cells

作　　者：黄银莉 周洪[1] 苏晓霞[1] 钟天宇 

机构地区：[1]西安交通大学口腔医学院正畸科,710004

出　　处：《实用口腔医学杂志》2017年第5期669-673,共5页Journal of Practical Stomatology

基　　金：陕西省"13115"科技创新工程重大科技专项计划项目(编号:2008ZDKG-65)

摘　　要：目的:比较人脐带Wharton’s Jelly来源间充质干细胞(human umbilical cord Wharton’s Jelly-derived mesechymal stem cells,h UCWJMSCs)与人牙周膜干细胞(periodontal mesenchymal stem cells,h PDLSCs)成骨分化能力。方法:体外培养h UCWJMSCs和h PDLSCs。MTT法检测细胞增殖情况;成骨诱导后测定细胞的ALP活性,茜素红染色检测细胞矿化能力,Real-time PCR分析OPN和Runx2基因的表达。结果:hUCWJMSCs增殖能力高于hPDLSCs;经矿化诱导后hPDLSCsALP表达、矿化结节形成高于hUCWJMSCs(P<0.05);Runx2在hPDLSCs中表达高于hUCWJMSCs(P<0.05);而hUCWJMSCs中OPN表达高于hPDLSCs(P<0.05)。结论:h UCWJMSCs、hPDLSCs均具有成骨分化能力,hPDLSCs成骨分化能力较强。Objective： To compare the osteogenesis ability between human umbilical cord Wharton＇ s Jelly-derived mesenchymal stem cells（hUCWJMSCs） and human periodontal ligament mesenchymal stem cells （hPDLSCs） in vitro. Methods： hUCWJMSCs and hP- DLSCs were in vitro cultured. The cell proliferation capacity was examined by MTT assay. After osteogenesis induction culture, ALP activity of the cells was determined, minerialization was observed by alizarin red staining, OPN and Runx2 mRNA expression was analyzed by Real-time PCR. Results： hUCWJMSCs grew faster than hPDLSCs. After osteogenic differentiation induction, hPDLSCs group showed higher ALP level, more mineralized nodule formation and higher Runx2 expression compared with hUCWJMSCs group（P 〈 0. 05） ; while the OPN expressed higher in hUCWJMSCs than in hPDLSCs （ P 〈 0.05 ）. Conclusion： hUCWJMSCs and hPDLSCs have osteogenesis differentiation potential, hPDLSCs are more osteogenetic.

关 键 词：人脐带Wharton’s Jelly间充质干细胞(hUCWJMSCs) 人牙周膜干细胞(hPDLSCs) 成骨分化 

分 类 号：R782[医药卫生—口腔医学]