瘤苗联合CpG ODN对白血病小鼠Treg细胞的影响  

Effect of tumor vaccine combined with CpG ODN on Treg cells in mice with leukemia

作　　者：杨云[1] 白菊[1] 何爱丽[1] 王芳侠[1] 沈莹[1] YANG Yun BAI Ju HE Aili WANG Fangxia SHEN Ying(Department of Hematology, Second Affiliated Hospital of Xi＇ an Jiaotong University, Xi ＇ an 710004, China)

机构地区：[1]西安交通大学第二附属医院血液科,西安710004

出　　处：《山西医科大学学报》2017年第9期930-935,共6页Journal of Shanxi Medical University

基　　金：西安市科技计划基金资助项目(SF1418)

摘　　要：目的探讨红白血病FBL-3细胞瘤苗联合CpG 1826治疗白血病小鼠时调节性T细胞(regulatory T cells,Treg)的变化及其意义。方法 50只小鼠随机分为对照组、PBS组、瘤苗组、CpG 1826组和瘤苗加CpG 1826组,皮下注射红白血病FBL-3细胞建立小鼠白血病模型,丝裂霉素C灭活FBL-3细胞制备瘤苗。PBS组、瘤苗组、CpG 1826组和瘤苗加CpG 1826组分别利用PBS、瘤苗、CpG 1826和瘤苗加CpG 1826进行治疗,而对照组不进行任何处理。流式细胞术检测小鼠脾细胞中Treg细胞百分率,逆转录聚合酶链反应(reverse transcription polymerase chain reaction,RT-PCR)测定Foxp3 mRNA的表达,ELISA法检测血清中IL-10水平。结果与对照组小鼠相比,PBS组、瘤苗组小鼠CD4^+Fox P3^+Treg细胞百分率、Foxp3 mRNA的表达水平、血清中IL-10水平明显增高,差异具有统计学意义(P<0.05),而PBS组与瘤苗组小鼠之间差异无统计学意义(P>0.05)。CpG 1826组、瘤苗联合CpG 1826组小鼠CD4^+FoxP3^+Treg细胞百分率、Foxp3 mRNA的表达水平、血清中IL-10水平比PBS组及瘤苗组明显下降,差异具有统计学意义(P<0.05)。结论瘤苗联合CpG 1826治疗小鼠白血病时Treg细胞减少,Treg细胞在白血病及免疫治疗中可能发挥了重要作用。Objective To explore the effect of tumor vaccine combined with CpG ODN on change of regulatory T（ Treg） cells in mice with leukemia. Methods Fifty mice were randomly divided into control group,PBS group,tumor vaccine group,CpG 1826 group and tumor vaccine with CpG group（ combination group）.The animal model of mouse with leukemia was established by subcutaneous injection with erythroleukemia FBL-3 cells. Tumor vaccine was prepared by mitomycin C inactivating FBL-3 cells. The mice in PBS group,tumor vaccine group,CpG 1826 group and combination group were treated with PBS,tumor vaccine,CpG 1826 and tumor vaccine＋CpG,respectively. The mice in control group received no treatment. The percentages of Treg cells were detected by flow cytometry and the expression levels of Foxp3 mRNA were detected by reverse transcription polymerase chain reaction（ RT-PCR）. Serum IL-10 levels were measured by enzyme-linked immunosorbent assay（ ELISA）. Results Compared with normal control group,the percentages of CD4～＋FoxP3～＋Treg cells,the mRNA expression of Foxp3 and the serum IL-10 levels in PBS group and tumor vaccine group were statistically increased（ P〈0. 05）,but there was no significant difference between PBS group and tumor vaccine group（ P〈0. 05）. The percentage of CD4～＋FoxP3～＋Treg cells,the mRNA expression of Foxp3 and the serum IL-10 levels in CpG 1826 group and combination group were statistically lower than those in PBS group and tumor vaccine group（ P〈0. 05）. Conclusion The tumor vaccine combined with CpG ODN may reduce Treg cells in mice with leukemia,and Treg cells may play roles in lukemia and immunotherapy.

关 键 词：白血病 免疫治疗 瘤苗 调节性T细胞 

分 类 号：R733.7[医药卫生—肿瘤]