浊点萃取-纳米金探针联用可视化检测鱼肉中的Hg^(2+)  

Visual detection of mercury(Hg^(2+)) in fish by cloud point extraction-gold nanoparticle probe

作　　者：王正 王晓伟 郭蒙京[2] 李倩[3] 薛颖 沙博郁 赵婕 WANG Zheng WANG Xiao-Wei GUO Meng-Jing LI Qian XUE Ying SHA Bo-Yu ZHAO Jie(Center for Preventive Medicine Research of Beij＇ing, Beijing 100013, China Center for Disease Prevention and Control of Xicheng District, Beijing 100120, China Center for Disease Prevention and Control of Daxing District, Beijing 102600, China)

机构地区：[1]北京市预防医学研究中心,北京100013 [2]北京市西城区疾病预防控制中心,北京100120 [3]北京市大兴区疾病预防控制中心,北京102600

出　　处：《食品安全质量检测学报》2017年第8期3197-3201,共5页Journal of Food Safety and Quality

基　　金：首都卫生发展科研专项(2014-3-1012)~~

摘　　要：目的建立一种浊点萃取与纳米金探针联用快速可视化检测鱼肉中Hg^(2+)的方法。方法修饰的纳米金粒子与Hg^(2+)的特异性络合,经浊点萃取进入有机相,并出现可肉眼观察的红色沉淀。根据有机相中沉淀量的多少或颜色深浅可判定Hg2+的浓度。结果 Hg^(2+)的浓度在1~15μg/L范围内,沉淀量和沉淀颜色与溶液中的Hg^(2+)的浓度呈正相关。本方法肉眼可识别的Hg^(2+)的浓度最低为2μg/L。结论本方法对Hg^(2+)的选择性好、灵敏度较高、检测时间短、操作简便,可用于鱼肉中Hg^(2+)的的快速定性分析。Objective To establish a visual detection method for tract mercury （Hg^2＋） in real fish samples, based on the cloud point extraction and gold nanoparticle （AuNP） probe techniques. Methods Modified and purified AuNP was specifically complexed with Hg^2＋ in the basified digestion solution of fish sample. The complex was then extracted by cloud point extraction and the red deposits were appeared in the Triton X-114. According to the amount or color of precipitation in organic phase, Hg^2＋ concentration could be determined. Results There was a positive correlation of the concentration of Hg^2＋ and amount of red deposit in the range of 1～15 μg/L Hg^2＋ in the basified digestion solution of fish samples. The lowest concentration which could be distinguished by naked eye was 2 μg/L. Conclusion This method is rapid, efficient and convenient with good selectivity and high sensitivity, which can be used for rapid qualitative analysis of Hg^2＋ in fish samples.

关 键 词：浊点萃取 纳米金探针 可视化检测 汞 鱼 

分 类 号：O657.3[理学—分析化学] TS254.7[理学—化学]