α-烯醇化酶联合CYFRA21-1、CA125在恶性胸腔积液诊断中的应用  

Application of alpha-enolase combined with CYFRA21-1 and CA125 in diagnosis of malignant pleural effusion

作　　者：李汝芳 张剑青[1] 王旭明[1] 武江海[1] 冯家钢[1] 赵芝焕[1] 

机构地区：[1]昆明医科大学第一附属医院呼吸内二科,650032 [2]云南省第一人民医院呼吸内科

出　　处：《实用医学杂志》2017年第18期3114-3118,共5页The Journal of Practical Medicine

基　　金：云南省卫生科技计划项目(编号:2014NS165)

摘　　要：目的通过比较恶性胸腔积液、结核性渗出性胸腔积液、类肺炎性胸腔积液患者α-烯醇化酶(ENO1)、CYFRA21-1、CA125浓度,探讨胸腔积液ENO1联合血清CYFRA21-1、CA125检测在恶性胸腔积液诊断中的价值。方法采用酶联免疫吸附实验(ELISA)检测胸腔积液ENO1浓度,化学发光法检测血清CA125浓度,磁微粒化学发光法检测血清CYFRA21-1浓度,计算ENO1联合CYFRA21-1、CA125在3组胸腔积液中的灵敏度、特异度。结果 (1)恶性胸腔积液组的ENO1浓度显著升高(P<0.001),ENO1在结核性渗出性胸腔积液与类肺炎性胸腔积液相比,差异无统计学意义(P>0.05);(2)ENO1联合血清CYFRA21-1、CA125检测在恶性胸腔积液中的灵敏度及特异度分别为94%和74%、98%和48%。结论 ENO1浓度在恶性胸腔积液中升高,ENO1联合血清CYFRA21-1、CA125检测可提高诊断恶性胸腔积液的灵敏度,提高恶性胸腔积液的诊断率。Objective To compare the concentrations of alpha - enolase （. ENO1 ） , CYFRA21 - 1, and CA125 in the patients with malignant pleural effusion, tuberculous exudative pleural effusion, or parapneumonia pleural effusion. To explore the clinical value of ENO1 in pleural effusion combined with serum CYFRA21-1 and CA125 in diagnosis of malignant pleural effusion. Methods Enzyme-linked immunosorbent assay （ELISA） was used to detect the concentration of ENO1 in pleural effusions. The concentrations of CA125 and CYFRA21-1 in the blood samples were measured using chemiluminescence and magnetic particle-based chemiluminescence respective- ly. The sensitivity and specificity of ENO1 combined with serum CYFRA21-1 and CA125 detection were calculated. Results The concentration of ENO1 in malignant pleural effusion group was significantly increased （P 〈 0.001 ） ; the concentrations of ENO 1 did not differ significantly between tuberculous exudative pleural effusion and parapneu- monia pleural effusion （P 〉 0.05）. The sensitivity and specificity of ENO1 combined with serum CYFRA21-1 and CA125 detection in malignant pleural effusion were 94% and 74%, 98% and 98%, respectively. Conclusions ENO1 combined with serum CYFRA21-1 and CA125 detection can improve the sensitivity of diagnosis of malignant pleural effusion and enhance the diagnostic rate of malignant pleural effusion.

关 键 词：α-烯醇化酶 胸腔积液 CYFRA21-1 CAL25 

分 类 号：R734.2[医药卫生—肿瘤]