老龄鼠海马星形胶质细胞IL-1β持续表达桥接急性和慢性神经炎症反应  

Prolonged expression of IL-1β in hippocampusal astrocytes bridges acute and chronic neuroinflammarion in aged rats

作　　者：付惠群[1] 阚敏慧[1] 范隆[1] 赵磊[1] 王天龙[1] 

机构地区：[1]首都医科大学宣武医院麻醉科,北京100053

出　　处：《中国医师杂志》2017年第9期1345-1349,共5页Journal of Chinese Physician

基　　金：北京市卫生系统高层次卫生技术人才培养计划（2013-2-033）;首都医科大学宣武医院创新团队培养资助项目

摘　　要：目的观察急性神经炎症反应向慢性神经炎症反应转变中IL-1β持续表达的细胞来源，为阻断神经炎症反应的靶向治疗提供依据。方法老龄鼠单次腹腔注射LPS（2mg／kg，LPS组）或0．9％生理盐水（对照组）。ELISA和RT-PCR测定海马区IL-1β及其基因表达。组织免疫荧光双标定位海马区IL-1β的细胞来源。结果老龄鼠海马区胶质纤维酸性蛋白（GFAP）和离子钙接头蛋白抗原（IBA-1）阳性细胞在LPS治疗后1d增加（P〈0．05），30d逐渐恢复到正常状态。这些变化与海马区IL-1β mRNA及星形胶质细胞源IL-1β表达升高一致。LPS注射后任何时间点均未观察到IL-1β在小胶质细胞及神经元内表达。结论LPS诱导胶质细胞延迟激活及星形胶质细胞源性IL-1β持续表达，后者可能在急性神经炎症反应向慢性神经炎症反应转变中扮演了重要角色。Objective To investigate which cells persistantly express interleukin （IL）-1β during the transition of acute neuroinflammaiton to chronic neuroinflammaiton and therefore to provide the evidences of targeted therapy for blocking neuroinflammation. Methods A single does of lipopolysaccharide （LPS） （2 mg/kg,LPS group） or 0. 9% saline （Control group） was intraperitoneally injected in aged rats. Level and gene expression of IL-1β in hippocampual tissue were measured by enzyme-linked immunosorbent assay （ELISA） and real time polymerase chain reaction （RT-PCR）, respectively. Cells secreted IL-1β in hippocampus was detected via double fluorescence. Results Glial fibrillary acidic protein （GFAP） and ionized calcium-binding adaptor molecule-1 （ IBA-1 ） positive cells were significantly increased day 1 （ P 〈 0. 05 ） and latee on, gradually return to base level 30 days after LPS exposure. These changes were consistent with mRNA expressions of IL-1β and astrocytes-derived IL-1β in hippocampus. Neither IL-1β expressed in microglia nor in neurons was observed at any time point following LPS-treated. Conclusions LPS induces prolonged activation of glial cells and sustained expression of astrocytes-derived IL-1β, which may play an important role in the transition of acute neuroinflammation to chronic neuroinflammation.

关 键 词：海马/细胞学/代谢 星形细胞/代谢 白细胞介素1B/代谢 神经系统疾病/代谢 炎症/代谢 衰老 

分 类 号：R741[医药卫生—神经病学与精神病学]