胃液microRNA-133a在胃癌诊断和预后评估中的作用  

Low microRNA-133a level in gastric juice forebodes unfavorable progression and prognosis of gastric cancer

作　　者：邵娟[1,2] 孔桂美 史明仪[1] 卜平[1] SHAO Juan KONG Gui-mei SHI Ming-yi BO Ping(Department of Digestive Diseases Research, Medical College of Yangzhou University, Jiangsu Yangzhou 225001, China Sanitary Corps of Yangzhou City Detachment, Jiangsu Provincial Corps of Chinese People＇ s Armed Police Force, Jiangsu Yangzhou 225003, China)

机构地区：[1]扬州大学医学院消化病研究室,江苏扬州225001 [2]武警江苏总队扬州市支队卫生队,江苏扬州225001

出　　处：《解剖学报》2017年第5期550-555,共6页Acta Anatomica Sinica

基　　金：国家自然科学基金(81673736)

摘　　要：目的探讨胃液中microRNA-133a(MiR-133a)低表达是否与胃癌的进展和预后相关。方法采集236例患者的胃液和胃黏膜样品,包括34例健康对照和202例胃癌样本,调查胃液中MiR-133a水平与胃癌的进展以及预后评估之间的相关性。通过Transwell小室实验和划痕实验观察胃癌细胞侵袭和迁移能力的变化。结果胃癌患者胃液和癌组织中的MiR-133a水平显著下调,下调幅度与肿瘤分级、T分期和远处转移存在相关性。MiR-133a可显著抑制SGC-7901胃癌细胞的侵袭和迁移能力。结论胃液中MiR-133a低表达与胃癌发展进程和不良临床后果呈现较高相关性,提示胃液中MiR-133a水平可以作为该疾病的进展和预后的鉴别指标。Objective We previously reported that the microRNA-133a （MiR-133a） levels in gastric juice and carcinoma tissues of patients with gastric cancer were significantly down-regulated, the current research surveyed whether low MiR-133a levels in gastric juice are related to the progression and prognosis of gastric cancer. Methods The gastric juice and mucosa samples were collected from an independent set of 236 masked pattern： 34 from healthy donors and 202 from patients with gastric cancer. In addition, cell invasion and migration abilities were observed with Transwell invasion assay and wound healing migration assay. Results MiR-133a levels in gastric juice and carcinoma tissues of patients with gastric cancer were significantly downregulated, and associated with advanced tumor grade, T stage and presence of metastasis. MiR-133a significantly inhibited the invasion and migration abilities of gastric cancer cells. The low MiR-133a expression in gastric juice was taken as a poor prognostic marker of both overall and disease-free survival in patients with gastric cancer. Conclusion These findings justify that low MiR-133a expression in gastric juice strongly associates with advanced tumor progression and unfavorable clinical outcome of patients with gastric cancer, and might be used as a useful tool to estimating progression and prognosis of this disease.

关 键 词：microRNA-133a 胃液 胃癌 Transwell实验 划痕实验 人 

分 类 号：R735[医药卫生—肿瘤]