茶树对铅胁迫的响应及其组织铅化学形态变化研究  

Biological Responses and Pb Speciation in Tissues After Pb Exposure of Tea Plants

作　　者：叶江华[1,2] 吴承祯 贾小丽[1,3] 陈晓婷[4,3] 李远华[2] 王飞权[2] 卢莉 郑茂钟 胡永乐 张清旭[4] 王海斌[4,3] 

机构地区：[1]福建省生态产业绿色技术重点实验室(武夷学院),福建武夷山354300 [2]武夷学院茶与食品学院,福建武夷山354300 [3]福建农林大学,福建省农业生态过程与安全监控重点实验室,福建福州350002 [4]龙岩学院生命科学学院,福建龙岩364012

出　　处：《热带作物学报》2017年第9期1607-1613,共7页Chinese Journal of Tropical Crops

基　　金：国家重点研发计划(No.2016YFD0200900);教育部博士后科学基金(No.2016M600493);福建省自然科学基金青年基金(No.2017J05057)福建省生态产业绿色技术重点实验室开放课题基金资助项目(No.WYKF2016-6);福建省“2011协同创新中心”中国乌龙茶产业协同创新培育专项(No.201310);福建省中青年教师教育科研项目(No.JAT160504);武夷学院“人才引进项目”(No.YJ201701);武夷学院科研基金资助项目(No.XD201502)

摘　　要：为了分析不同茶树对Pb(铅)胁迫的响应及其组织中不同化学形态Pb的累积特性,本研究采用盆栽种植法,探讨铁观音、肉桂2种茶树在不同浓度Pb胁迫下,茶树叶片光合生理指标,包括光合作用速率和叶绿素含量,以及茶树组织Pb含量和不同化学形态Pb含量的变化。结果表明,随着Pb浓度的增加,茶树光合生理指标呈现下降趋势,且与对照相比,铁观音下降的幅度高于肉桂。同一Pb胁迫浓度下,茶树组织中Pb含量表现为根>叶。不同化学形态Pb含量分析结果表明,随着Pb胁迫浓度的升高,2种茶树根部可交换态Pb、碳酸盐结合态Pb和残留态Pb含量百分比呈现下降趋势,而有机结合态Pb和铁锰氧化态Pb含量呈现上升趋势;2种茶树叶片碳酸盐结合态Pb、可交换态Pb、铁锰氧化态Pb和有机结合态Pb百分含量呈现上升趋势,而残留态Pb百分含量呈现下降趋势。进一步分析发现,在Pb胁迫下,不同的茶树表现出解毒模式的差异性。In order to analyze the physiological responses of different tea trees to Pb stress and accumulation characteristics of Pb with different chemical forms in different tissues of tea trees, photosynthesis rate, chlorophyll content, Pb content in different tissues and Pb content with different chemical forms of Tieguanyin and Rougui tea plants were measured under Pb stess with different concentrations in pot culture. Results showed that the photosynthesis rate and chlorophyll content of tea plants descended with the increase of Pb stress concentration,but the falling range of Tieguanyin was higher than Rougui as compared with the control. At the same concentration of Pb stress, Pb contents in tea plant roots and leaves performed root leaf. Analysis of Pb contents with different chemical forms showed that the content percentages of exchangeable Pb, carbonate-bound Pb, residual Pb from two tea tree roots had downward trends, while organic-bound Pb and Pb with Fe-Mn oxidation state had upward trends as stress concentration increased. Content percentages of exchangeable Pb, carbonate-bound Pb, organic-bound Pb and Pb with Fe-Mn oxidation state in the leaf appeared to be rising, while residual Pb declined.Further analysis indicated that detoxification patterns of different tea plant varieties exhibited variance under Pb stress.

关 键 词：茶树 PB胁迫 化学形态 光合生理指标 

分 类 号：S571.1[农业科学—茶叶生产加工]