HPLC波长切换法同时测定消痰益康(糖浆)中6种成分的含量  

Simultaneous determination of six components in Xiaotan Yikang syrup by HPLC switching wavelength method

作　　者：王华 

机构地区：[1]解放军天津疗养院药剂科,天津300191

出　　处：《实用药物与临床》2017年第9期1067-1070,共4页Practical Pharmacy and Clinical Remedies

摘　　要：目的建立同时测定消痰益康(糖浆)中多成分的定量分析方法。方法采用HPLC波长切换法同时测定原儿茶醛、儿茶素、表儿茶素、金丝桃苷、槲皮素和杜鹃素的含量。色谱柱为Agilent TC-C18(250 mm×4.6 mm,5μm);以甲醇-0.04%冰醋酸溶液为流动相,按规定进行梯度洗脱;流速0.9 mL/min;检测波长分别为280 nm(原儿茶醛、儿茶素和表儿茶素)、360 nm(金丝桃苷和槲皮素)和299 nm(杜鹃素);柱温30℃。结果原儿茶醛、儿茶素、表儿茶素、金丝桃苷、槲皮素和杜鹃素分别在7.290~145.8、2.550~51.00、13.76~275.2、6.470~129.4、2.660~53.20、3.880~77.60μg/m L范围内与峰面积呈良好的线性关系;平均加样回收率及相应的RSD分别为98.68%(1.35%)、99.27%(1.17%)、97.05%(0.58%)、99.84%(1.19%)、97.89%(0.95%)、98.78%(1.13%)。结论该方法可用于消痰益康(糖浆)的含量测定及质量控制。Objective To establish a method for the simultaneous determination of the multiple components in Xiaotan Yikang syrup. Methods The contents of protocatechuic aldehyde,（ ＋）-catechin,epicatechin,hyperoside,quercetin and farrerol were determined by HPLC gradient elution method. The Agilent TC-C18（ 250 mm × 4. 6 mm,5μm） chromatographic column was adopted; the mobile phase consisted of A（ methanol） and B（ 0. 04% glacial acetic acid solution） with the column temperature being 30 ℃ and gradient elution; UV detection wavelength was set at 280 nm [protocatechuic aldehyde,（ ＋）-catechin,epicatechin],360 nm（ hyperoside,quercetin） and 299 nm（ farrerol） with the flowrate of 0. 9 m L/min. Results Protocatechuic aldehyde,（ ＋）-catechin,epicatechin,hyperoside,quercetin and farrerol,within the scope of 7. 290 ~ 145. 8,2. 550 ~ 51. 00,13. 76 ~ 275. 2,6. 470 ~ 129. 4,2. 660 ~ 53. 20 and 3. 880 ~77. 60 μg/m L,had a good linear relationship,respectively. The average recoveries and the corresponding RSD were98. 68%（ 1. 35%）,99. 27%（ 1. 17%）,97. 05%（ 0. 58%）,99. 84%（ 1. 19%）,97. 89%（ 0. 95%） and 98. 78%（ 1. 13%）,respectively. Conclusion This method can be used for content determination and quality control of Xiaotan Yikang syrup.

关 键 词：消痰益康(糖浆) 原儿茶醛 儿茶素 表儿茶素 金丝桃苷 槲皮素 杜鹃素 波长切换法 中成药 多成分测定 

分 类 号：O657.72[理学—分析化学] R286.0[理学—化学]