曲酸对关中黑猪精液常温保存的影响  

Effects of Kojic Acid on the Quality of Boar Semen of Guanzhong Black Pig during Room Temperature Preservation

作　　者：侯震坤[1] 魏宁[1] 刘兆军[1] 谢景兴[1] 杨公社[1] 庞卫军[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《养猪》2017年第5期37-40,共4页Swine Production

基　　金：国家生猪产业技术体系(CARS-36);西北农林科技大学试验示范站(基地)科技成果推广项目(TGZX2015-18)

摘　　要：研究将抗氧化剂曲酸(Kojic acid)应用到关中黑猪精液的常温保存中,通过渗透压、精子活率、质膜完整率、顶体完整率和总抗氧化能力(T-AOC)等指标检测,探究曲酸对精子的保护作用,为关中黑猪精液稀释粉的研发提供技术支持。试验结果显示,BTS稀释液中添加0.1 g/L浓度以内的曲酸能够有效提高精子质膜、顶体完整性,提升精子的总抗氧化能力。其中0.04 g/L浓度组保存效果最好,保存第5天时精子活率为61.45%,可将精子有效保存时间延长2 d,0.06 g/L浓度组与之差异不显著(P>0.05)。综上所述,在BTS液中添加0.04 g/L曲酸能够对精子常温保存起到最佳的抗氧化保护效果。研究对解决猪精液常温保存中精子的氧化损伤问题具有重要参考意义,同时为自主研发稀释粉产品提供试验依据。In this study,antioxidant kojic acid were applied to the preservation of Guanzhong black pig semen at room temperature.Osmotic pressure,sperm viability,plasma membrane integrity,acrosome integrity and total antioxidant capacity（T-AOC） and some other detection indexes were experimented to explore the efficacy of kojic acid on the protection of semen,this study was aimed to provide a theoretical support for the research of dilution powder for Guanzhong black pig semen.The following are the main results in this study：BTS extenders solution with less than 0.1 g/L kojic acid can significantly improve the sperm plasma membrane,acrosome integrity,enhance the total antioxidant capacity of sperm（P〈0.05）,especially 0.04 g/L group has the best performance for the sperm viability was 61.45% at the 5th day which can prolong the sperm storage time for another 2 days.In addition,the sperm preservation effect of 0.06 g/L group was not significantly different with 0.04 g/L group（P〉0.05）.In summary,adding 0.04 g/L kojic acid in BTS solution had the best antioxidation protection effect on sperm preservation at room temperature.The results of this study are of great significance to solve the problem of sperm oxidative damage in porcine semen preservation at room temperature,and provide experimental basis for independent diluted powder research.

关 键 词：曲酸 关中黑猪 猪精液 常温保存 

分 类 号：S828.3[农业科学—畜牧学]