山东省动物源沙门氏菌MLST和血清分型与分布研究  

Distribution and typing of animal-derived Salmonella with MLST and serotype

作　　者：赵翠 张庆[2] 郭树源[3] 李璐璐[2] 刘玉洁 王永明 常维山[3] 刘玉庆[2] 

机构地区：[1]山东省泰安市动物卫生监督所,泰安271000 [2]山东省农业科学院畜牧兽医研究所,泰安250100 [3]山东农业大学动物科学院,济南271018 [4]山东省日照市动物疫病预防控制中心,日照276800 [5]山东华宏生物工程有限公司,济南256600

出　　处：《中国人兽共患病学报》2017年第9期793-799,共7页Chinese Journal of Zoonoses

基　　金：山东省农业科学院科技创新工程(CXGC2016A10);山东省农业重大应用技术创新项目(SDAIT-11-09)~~

摘　　要：目的比较动物源沙门氏菌多位点序列分型(MLST)与血清分型的差别,获得动物源沙门氏菌在山东省的分布规律。方法从山东部分地区分离78株鸡源沙门氏菌、56株鸭源沙门氏菌和20株猪源的沙门氏菌,PCR扩增七段沙门氏菌内部保守的片段序列进行多位点序列分型,同时用玻片凝集法分析其血清型。结果血清分析结果表明,鸡源沙门氏菌检出6种血清型,其中肠炎沙门氏菌占88.5%、印第安纳沙门氏菌占5.1%、汤卜逊沙门氏菌占2.6%、鼠伤寒沙门氏菌占1.3%、山夫登堡沙门氏菌占1.3%、阿格玛沙门氏菌占1.3%;鸭源沙门氏菌检出2种血清型,其中肠炎沙门氏菌占67.9%、鼠伤寒沙门氏菌占32.1%;猪源沙门氏菌检出3种血清型,其中鼠伤寒沙门氏菌占65%、德贝儿沙门氏菌占20%、肠炎沙门氏菌占15%。通过MLST分型,鸡源检出7种ST型:ST11、ST19、ST26、ST128、ST14、ST17和New1;鸭源检出3种ST型:ST11、ST19和New2;猪源检出3种:ST34、ST40和ST3007。结论整体来看,分离菌的血清型和ST型数量比较接近,二者分型能力相近。To compare the discriminatory ability of multilocus sequence typing and serotype of animal-derived Salmonella and find its distribution in Shandong Province, 78 chicken-origin, 56 duck-origin and 20 swine-origin Salmonella were separa- ted from some regions of Shandong Province. Seven conserve sequences of Salmonella were PCR-amplified for MLST and slide agglutination test for serotyping. Results showed that by serotyping, 6 serotypes were identified from chicken-origin Salmonel- la, including 88.5% S. enteritidis, 5.1% S. indiana, 2.6% S. thompson, 1.3% S. typhimurium, 1.3% S. senftenberg, 1.3% S. agama. Two serotypes were identified from duck-origin Salmonella, including 67.9% S. enteritidis, 32.1% S. typhimurium. Three serotypes were identified from swine-origin Salmonella, including 65 % S. typhirnurium, 20% S. derby, and 15% S. enteritidis. By MLST typing, seven ST types were identified from chicken-origin Salmonella： STll, ST19, ST26, ST128, ST14, ST17 and Newl. Three ST types were identified form duck-origin Salmonella： ST11, ST19 and New2.Three ST types were identified from swine-origin Salmonella： ST34, ST40 and ST3007. Overall, the types identified with two methods were closed, so MLST and sero- type have similar discriminatory ability.

关 键 词：沙门氏菌 血清型 MLST 分型 

分 类 号：R183.1[医药卫生—流行病学]