采用离子色谱检测肉及肉制品中多聚磷酸盐  

Determination of polyphosphates in meat and meat products by ion chromatography

作　　者：刘佳[1,2] 沈娟[1,2] 詹玮[1] 张云 赵彦杰 王玮 

机构地区：[1]南京农业大学食品科技学院,江苏南京210095 [2]南京农业大学农业部肉及肉制品质量监督检验测试中心(南京),江苏南京210095

出　　处：《食品与发酵工业》2017年第9期215-218,共4页Food and Fermentation Industries

基　　金：农产品产地收贮运环节中未知危害因子识别与已知危害因子安全性评估项目(GJFP2015012);南京农业大学SRT计划项目(1618A20)

摘　　要：采用离子色谱技术同时检测肉及肉制品中的正磷酸盐、焦磷酸盐、三聚磷酸盐和三偏磷酸盐成分。对建立的方法进行检出限、定量限和最佳线性范围的确定,并进行加标回收和精密度测定,以及实际应用效果评价。4种磷酸盐分离度良好,正磷酸盐、焦磷酸盐、三聚磷酸盐、三偏磷酸盐检出限分别为0.01、0.15、0.15、0.15mg/L,定量限分别为0.05、0.5、0.5、0.5 mg/L,分别在0.05~200、0.2~200、0.2~200、0.2~200 mg/L浓度内,呈良好线性关系。在3个浓度水平加标,其中正磷酸盐1 000、2 000、5 000 mg/kg,焦磷酸盐、三聚磷酸盐和三偏磷酸盐50、100、200 mg/kg,加标回收率为83.2%~101.9%,相对标准偏差(relative standard deviation,RSD)小于2.02%。用建立的方法对市售产品进行检测分析,结果可以满足市售肉及肉制品样品的检测需求。A rapid and accurate method for simultaneous determination of orthophosphate, pyrophosphate, tripolyphosphate and trimetaphosphate in meat and meat products were developed by using ion chromatography （IC）. The limitation of the detection, the quantification and the best linear range of the method were determined ; the standard recovery and precision were also found, and the effect of the actual application was evaluated. Four kinds of phosphates can be easily separated and the limits of detections （LODs） were 0.01, 0.15, 0.15, and 0.15 mg/L for orthophosphate, pyrophosphate, tripolyphosphate and trimetaphosphate, respectively. The limitation of quantifications （LQDs） were 0.05, 0. 5, 0.5, 0.5 mg/L for orthophosphate, pyrophosphate, tripolyphosphate and trimetaphosphate, respectively. The average recoveries of spiked samples were between 83.2% -101.9% , with the relative standard deviations （RSD） less than 2.02%. Meat and meat products in the market were detected using this method. The method is convenient, fast and accurate, and can be applied for the rapid and accurate detection of polyphosphates in different kinds of meat and meat products.

关 键 词：肉及肉制品 离子色谱 多聚磷酸盐 

分 类 号：O657.7[理学—分析化学] TS251.7[理学—化学]