溶葡球菌酶高效表达与应用  

Progress on High Efficient Expression and Application of Lysostaphin

作　　者：王曦 陈熙明[2] 浦铜良[1] WANG Xi CHEN Xi-ming PU Tong-liang(School of Life Sciences, Lanzhou University, Lanzhou 730000, China Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, China)

机构地区：[1]兰州大学生命科学学院,兰州730000 [2]中国科学院西北生态环境研究院,兰州730000

出　　处：《中国生物工程杂志》2017年第9期118-125,共8页China Biotechnology

摘　　要：溶葡球菌酶(lysostaphin,Lys)是采用基因克隆技术使溶葡球菌酶基因实现外源表达所产生的蛋白质。它是Zn2+依赖的金属蛋白酶,具有肽链内切酶活性,能专一性地水解葡萄球菌细胞壁Gly五肽桥联,使金黄色葡萄球菌(特别是MRSA)细胞壁破裂,达到溶菌杀菌作用,而不产生耐药性。作为一种抗菌剂,在兽药与临床等领域具有巨大的应用潜力。综述对溶葡球菌酶的来源、作用机制、不同表达系统及前景与展望进行综述。Lysostaphin（Lys） is usually exogenously expressed in varieties of cells or organisms with gene cloning technology. Lys is a Zn^2＋ metalloenzyme and a glycylglycinen endopeptidase specific to the pentaglycine cross-bridges of peptidoglycan in the cell walls of Staphylococcus aureus,especially,methicillin-resistant S.aureus（MRSA）,then showing an unique bacteriolytic activities. As a kind of efficient antibacterial agent,it has enormous application potentialities in the fields of veterinary drugs and clinical applications. It was reviewed the origins and lysing mechanism of the Lys, the varieties of exogenously gene expression systems. And the developmental situation and prospects of Lys applications were also discussed.

关 键 词：溶葡球菌酶 金黄色葡萄球菌 高效表达 应用 

分 类 号：Q814[生物学—生物工程]