血清高尔基体蛋白73联合甲胎蛋白诊断原发性肝癌的临床意义  

Clinical value of serum Golgi protein 73 combined with alpha-fetoprotein in the diagnosis of primary hepatic carcinoma

作　　者：张丽萍[1] 周慧芳[1] 米热尼沙.热米吐拉 刘雯[1] 许爱敏[1] 李泽泳 

机构地区：[1]新疆喀什地区第一人民医院检验科,新疆喀什844000

出　　处：《转化医学电子杂志》2017年第9期36-38,共3页E-Journal of Translational Medicine

基　　金：新疆自治区自然科学基金(2016D01C024)

摘　　要：目的:探讨高尔基体蛋白73(GP73)联合血清甲胎蛋白(AFP)诊断原发性肝癌的临床价值.方法:回顾分析230例原发性肝癌组患者、208例良性肝脏疾病患者、178例健康对照者的血清AFP、GP73检测结果,其中AFP测定采用电化学发光法,GP73测定采用酶联免疫吸附试验(ELISA).结果:血清AFP、GP73诊断原发性肝癌组的ROC曲线下面积(AUC)分别为0.787、0.903,血清GP73诊断原发性肝癌的AUC显著高于血清AFP(P<0.05).血清甲胎蛋白诊断原发性肝癌的cut-off值定为187.7μg/L,AFP检测的特异度为94.3%,敏感度为77.4%.血清GP73诊断原发性肝癌的cut-off值定为143.1μg/L,GP73检测的特异度为95.3%,敏感度为84.3%.PHC组GP73和AFP水平明显高于良性肝脏疾病组和健康对照组,差异有统计学意义(P<0.05).良性肝脏疾病组的血清GP73和AFP水平明显高于健康对照组(P<0.05).采用平行试验对血清AFP、GP73指标进行联合检测诊断原发性肝癌组的敏感度为94.7%(218/230),特异度为92.6%(358/386).联合检测诊断PHC组的敏感度明显高于单独检测血清AFP的敏感度(77.4%)和GP73的敏感度(84.3%)(P<0.05);而联合检测的特异度与单独检测血清AFP的特异度(95.3%)和GP73的特异度(94.3%)比较,差异无统计学意义(P>0.05).结论:单独检测血清AFP、GP73作为诊断原发性肝癌的敏感度较低,当两者联合时可以明显提高诊断灵敏度,从而有利于原发性肝癌组的诊断与鉴别诊断.AIM： To investigate the clinical value of Golgi protein 73（ GP73） combined with alpha-fetoprotein（ AFP） in the diagnosis of primary hepatic carcinoma（ PHC）. METHODS：The levels of serum AFP and GP73 in 230 cases of PHC,208 cases of benign hepatic diseases and 178 cases of healthy control were analyzed retrospectively. Level of AFP was determined by electrochemiluminescence,while level of GP73 was detected by enzyme-linked immunosorbent assay（ ELISA）. RESULTS： The area under the ROC curve（ AUC） of serum AFP and GP73 were0.787 and 0. 903 in the diagnosis of PHC respectively,in which the AUC of serum GP73 was significantly higher than that of serum AFP（ P〈0.05）. The cut-off value of AFP in the diagnosis of PHC was 187. 7 μg/L,specificity was 94. 3% and sensitivity was 77.4%. The cut-off value of GP73 in the diagnosis of PHC was 143. 1 μg/L, specificity was 95. 3% and sensitivity was84.3%. The levels of serum AFP and GP73 in control group were significantly higher than those of benign hepatic disease group and healthy controls, with statistically significant differences（ P〈0.05）. The levels of serum AFP and GP73 in benign hepatic disease group were significantly higher than those of healthy controls,with statistically significant differences（ P〈 0. 05）. The level of AFP combined with GP73 in the diagnosis of PHC was analyzed by parallel test. The sensitivity was 94. 7%（ 218/230）and specificity was 92. 6%（ 358/386）. The sensitivity of AFP combined with GP73 in the diagnosis of PHC was obviously higher than sensitivity of isolated AFP（ 77. 4%） or sensitivity of GP73（ 84.3%）（ P〈0.05）. There was no statistically significant difference between specificity through combined detection and specificity through single detection of AFP（ 95. 3%） or GP73（ 94. 3%）（ P〈0.05）. CONCLUSION： The sensitivity of serum AFP or GP73 detection alone was lower in the diagnosis of PHC,while the combined detection of both AFP and GP73 could increase the diagnostic

关 键 词：甲胎蛋白 高尔基体蛋白-73 原发性肝癌 

分 类 号：R735.7[医药卫生—肿瘤]