miRNA499在小细胞肺癌患者血清和肺泡灌洗液中的表达分析  

Expression of miRNA499 in serum and bronchoalveolar lavage fluid of patients with small cell lung cancer

作　　者：周凤丽[1] 冯定云[1] 邹小玲[1] 吴少珠[1] 刘燕飞[1] 

机构地区：[1]中山大学附属第三医院呼吸科,广东广州510630

出　　处：《转化医学电子杂志》2017年第9期39-41,45,共4页E-Journal of Translational Medicine

基　　金：广东省科技计划项目(2014A020212531)

摘　　要：目的:探讨miRNA499能否作为早期诊断小细胞肺癌(SCLC)的分子标志物.方法:收集26例SCLC患者的血清、肺泡灌洗液以及20例非肺癌呼吸疾病患者的血清,检测其RNA浓度、RT逆转录后c DNA的浓度;同时检测SCLC患者血清以及肺泡灌洗液中miRNA499的相对表达.结果:(1)SCLC患者血清和肺泡灌洗液中miRNA499的表达水平均明显升高;(2)非肺癌呼吸疾病患者几乎不表达或者不表达miRNA499.结论:miRNA499的检测有可能作为临床SCLC的早期诊断指标和基因治疗靶点.AIM： To determine whether miRNA499 can be used as a molecular marker for early diagnosis of small cell lung cancer SCLC. METHODS： The serum and bronchoalveolar lavage fluid from 26 patients with SCLC and serum of 20 cancer-free controls with respiratory disease were collected. The RNA concentration and the cDNA concentration after reverse transcription were detected. The expression of miRNA499 in serum and bronchoalveolar lavage fluid of patients with SCLC were also detected. RESULTS：（1） The expression levels of miRNA499 in serum and bronchoalveolar lavage fluid of patients with SCLC were significantly increased.（2）The miRNA499 gene was almost not expressed or not expressed in small cell lung cancer-free controls with respiratory disease. CONCLUSION： The miRNA499 may be used as the early diagnosis index and the target of gene therapy for SCLC.

关 键 词：肺癌 miRNA499 分子标志物 

分 类 号：R734.2[医药卫生—肿瘤]