成人跖疣人乳头瘤病毒分型及与临床特征的关系  

Relationship between human papiUomavirus genotypes in and clinical characteristics of adult plantar warts

作　　者：董强 杨番 任英云 夏天保[1] 尹萌萌 王泽锟 米霞[1] 刘军连[1] 曹晓佳[1] 刘建军[1] 葛芸 梁龙 吕世超[1] 

机构地区：[1]安徽医科大学解放军第306临床学院皮肤科,北京100101 [2]天津市武清区人民医院医疗美容科 [3]佛山市南海区第三人民医院皮肤科

出　　处：《中华皮肤科杂志》2017年第10期748-750,共3页Chinese Journal of Dermatology

摘　　要：目的检测成人跖疣乳头瘤病毒（HPV）型别，并探讨HPV分型与临床特征的关系。方法采用PCR扩增、基因测序和TA克隆技术检测221例跖疣患者疣体的HPV型，记录患者的年龄、病程、症状和疣体数。结果221例标本中，HPV阳性率为97％（215／221）。单一HPV型占96％（206／215），HPV27、2、57和7（Alpha属）分别占44．7％（92／206）、12．1％（25／206）、7．3％（15／206）和0．5％（1／206）；HPV65（Gamma属）占0．5％（1／206）；HPV1、63（Mu属）占33．5％（69／206）和1．5％（3／206）。HPV27、2和57三型感染者之间年龄、病程、疣体个数、疼痛发生率和性别比较，差异均无统计学意义。与HPV27感染者相比，HPV1感染者与年龄≤30岁、病程≤1年、疣体数≤2个和疼痛高发生率相关。结论HPV27、2、57和1是感染成人跖疣患者的主要HPV型。HPV型与患者的临床特征间存在相关性。Objective To investigate human papillomavirus （HPV） genotypes in adult patients with plantar warts, and to explore their relationship with clinical features of adult plantar warts. Methods PCR, gene sequencing and TA-eloning technique were performed to detect HPV types in 221 patients with plantar warts. Meanwhile, clinical data were recorded, including patients＇ age, disease duration, symptoms and number of warts. Results Of 22l specimens, HPV DNA was detected in 215, with tile HPV-positive rate being 97%. Single HPV infections were detected in 206 specimens （96%, 206/215）, among which, HPV types included HPV-27 （44.7%, 92/206）,-2 （12.1%, 25/206）,-57 （7.3%, 15/206）,-7 （Alpha genus; 0.5%, 1/206）,-65 （Gamma genus; 0.5%, 1/206）,-1 （33.5%, 69/206） and-63 （Mu genus; 1.5%, 3/206）. There were no significant differences in age, disease duration, number of warts, incidence of pain and gender among patients with HPV-27, -2 or -57 infections. Compared with patients with HPV-27 infection, patients with HPV- 1 infection were more related to age ≤30 years, disease duration ≤1 year, number of warts ≤ 2 and high incidence of pain. Conclusion HPV-27, -2, -57 and -1 are the main types in adult patients with plantar warts, and HPV types are correlated with clinical features of adult plantar warts.

关 键 词：乳头状瘤病毒科 基因分型技术 疣 PCR 临床特征 TA克隆 

分 类 号：R752[医药卫生—皮肤病学与性病学]