P2X7受体在匹罗卡品致癫痫大鼠海马中的表达与分布  

Expression and spatial distribution of P2X7 receptor in pilocarpine-induced epileptic rat hippocampus

作　　者：曾祥昌[1,2] 陈露露 周露萍[1,2] 罗伟 胡凯[3] 欧阳冬生 ZENG Xiangchang CHEN Lulu ZHOU Luping LUO Wei HU Kai OUYANG Dongsheng(Department of Clinical Pharmacology, Xiangya Hospital, Central South University, Changsha 410008 Institute of Clinical Pharmacology, Central South University,Hunan Key Laboratory of Pharmacogenetics, Changsha 410078 Department of Neurology, Xiangya Hospital, Central South University, Changsha 410008, China)

机构地区：[1]中南大学湘雅医院临床药理研究所,长沙410008 [2]中南大学临床药理研究所遗传药理学湖南省重点实验室,长沙410078 [3]中南大学湘雅医院神经内科,长沙410008

出　　处：《中南大学学报（医学版）》2017年第9期997-1002,共6页Journal of Central South University ：Medical Science

基　　金：国家自然科学基金(81301106);湖南省自然科学基金(14JJ4006;S2016J504B0999)~~

摘　　要：目的:探讨P2X7受体在匹罗卡品致癫痫大鼠海马中的动态表达与分布。方法:氯化锂-匹罗卡品诱导癫痫持续状态大鼠模型,于癫痫发作后第1,3,7,14,28天取大鼠海马,Western印迹检测P2X7受体蛋白表达,免疫组织化学分析P2X7受体在脑组织海马各区的分布。结果:大鼠腹腔注射匹罗卡品(33.9±12.3)min后出现双侧前肢阵挛伴后肢站立。P2X7受体蛋白表达在癫痫发作后1 d开始上调,3 d后逐渐下降,第7天时表达达到最低,随后持续上调至第28天,其中1,14,28 d时P2X7受体蛋白的表达水平与对照组相比,差异有统计学意义(P<0.05)。免疫组织化学结果显示P2X7受体在海马各区均有分布,癫痫发作后在海马DG和CA3区表达趋势与蛋白表达一致,而在CA1区表达无显著变化。结论:P2X7受体在癫痫持续状态大鼠中的表达变化呈时间依赖性和空间分布差异,P2X7可能参与慢性癫痫发生。Objective： To investigate the dynamic expression pilocarpine-induced epileptic rat hippocampus. and spatial distribution of P2X7 receptor in Methods： Status epilepticus （SE） model of rats was established by intraperitoneal injection with chloride lithium and pilocarpine. Rat brain tissue and hippocampus were collected at 1, 3, 7,14, 28 days after SE. The protein expression of P2X7 receptor in rat hippocampus was detected by Western blot. The distribution of P2X7 receptor in hippocampal sub-region was analyzed by immunohistochemistry. Results： Bilateral forelimb clonus appeared at （33.9＋12.3） min after intraperitoneal injection with pilocarpine. The protein expression of P2X7 receptor was increased at ld after SE, while it was decreased gradually from 3 d to minimum at 7 d, then it was elevated continuously to 28 d. Among them, the expression of P2X7 receptor was increased significantly at 1, 14 and 28 d post-SE （P〈0.05）. Immunohistochemical staining showed that P2X7 receptor was detected in all areas. The expression pattern of P2X7 receptor in hippocampal DG and CA3 area was consistent with protein expression, but its expression in hippocampal CA1 area was not significantly changed after SE. Conclusion： The expression of P2X7 receptor in post-SE hippocampus is in a time-dependent manner and spatial specificity. P2X7 receptor might be involved in the development of chronic epilepsy.

关 键 词：P2X7受体 癫痫持续状态 癫痫发生 

分 类 号：R742.1[医药卫生—神经病学与精神病学]