冻干保护剂对假单胞菌菌粉存活率的影响  

Effect of Cryoprotectant on the Survival Rate of Freeze-dried Pseudomonas sp. Powder

作　　者：李丽[1] 赵彭年[1] 朱希坤[1] LI Li ZHAO Peng-nian ZHU Xi-kun(Biotechnology and Pharmaceutical Research Laboratory, Shenyang Research Institute of Chemical Industry, Shenyang 110021)

机构地区：[1]沈阳化工研究院有限公司物与医药研究室,沈阳110021

出　　处：《生物技术通报》2017年第9期231-234,共4页Biotechnology Bulletin

摘　　要：保护剂的添加对提高假单胞菌冷冻干燥菌粉的存活率和贮藏稳定性有显著效果。设计实验对冻干保护剂进行筛选和优化研究,通过单因素实验和正交实验筛选出了最佳保护剂组合:脱脂乳粉10%,山梨醇3%,甘油1%,L-抗坏血酸钠2%,假单胞菌冻干后的存活率可达90%,菌粉25℃存放6个月,存活率为50.2%。The cryoprotectant presents remarkable effect on increasing the survival rate and stability of freeze-dried Pseudomonas sp.powder. Here single-factor and orthogonal experimental designs were conducted to screen the optimal combination of cryoprotectant. The resultsshowed that the survival rate reached 90% when the concentration of skim milk powder,sorbitol,glycerol,and L-sodium ascorbate were 10%,3%,1%,2%,and was 50.2% while stored for 6 months at 25℃.

关 键 词：假单胞菌 冻干保护剂 存活率 

分 类 号：Q93-33[生物学—微生物学]