ASPP2及P16^(INK4a)在食管癌中的表达及其与凋亡的关系  

Expression of ASPP2 and P16^(INK4a) and its relationship with apoptosis in esophageal carcinoma

作　　者：李凤玉[1] 王丽梅[2] 魏淑霞[1] 吕洋[3] 李秀娟[3] 李柔[3] 

机构地区：[1]中国人民解放军第251医院肿瘤内科,河北省张家口市075000 [2]内蒙古通辽市医院肿瘤放疗科,028007 [3]河北北方学院组胚教研室,河北省张家口市075000

出　　处：《实用医学杂志》2017年第19期3247-3250,共4页The Journal of Practical Medicine

基　　金：河北省科技厅计划项目(编号:15277707D)

摘　　要：目的探讨ASPP2、P16^(INK4a)在食管癌的表达与患者临床病理特征及凋亡的相关性。方法 SP免疫组化染色法检测112例食管癌及31例正常食管黏膜ASPP2、P16^(INK4a)的表达,分析它们与患者临床病理特征的相关性;在上述蜡块中随机选取37例食管癌及12例正常食管黏膜,采用原位缺口末端标记技术(TUNEL)检测上皮细胞的凋亡情况,分析凋亡阳性率与以上二标记物在食管癌表达的相关性。结果 ASPP2、P16^(INK4a)在食管癌与正常食管黏膜的表达差异有统计学意义,且二者异常表达均与食管癌组织学分级、淋巴结转移相关(P<0.05)。TUNEL检测显示正常食管黏膜与食管癌的凋亡阳性率差异有统计学意义,且其与ASPP2、P16^(INK4a)在食管癌的表达相关(P<0.05)。结论 ASPP2、P16^(INK4a)参与食管癌发生、发展及凋亡等过程,联合检测二者可辅助食管癌诊断并指导临床治疗。Objective To study the expression of ASPP2 andP16INK4a in esophageal carcinoma and their relationship to the apoptosis and related clinicopathological characteristics. Methods Immunohistochemistry S-P method was used to examine the expression of ASPP2 and P16INK4a in the pathological specimens of 112 esophageal carcinoma cases and 31 cases of normal esophageal mucosa. TUNEL was also employed to detect the rate of apopto- sis in 37 esophageal carcinoma cases and 12 cases of normal esophageal mucosa. Results The difference between the expression of ASPP2 and P16INK4a in esophageal carcinoma and normal esophageal mucosa was statistically signif- icant （P 〈 0.05 ）. Their abnormal expressions were all related to lymph node metastasis and differentiation degree （P 〈 0.05 ）. The difference between the positive rate of apoptosis in esophageal carcinoma and normal esophageal mucosa was statistically significant （P 〈 0.05 ）. The abnormal expression of ASPP2 and P16INK4a was all related to apoptosis in esophageal carcinoma. Conclusions The different expression of ASPP2 and P16INK4a may cooperativelyplay a role in differentiation degree, lymph nodes metastasis and apoptosis in esophageal carcinoma. Co-examina- tion of them may be useful for the diagnosis and guiding the clinical treatment in esophageal carcinoma.

关 键 词：食管癌 ASPP2 P16INK4A 免疫组织化学 原位缺口末端标记技术 凋亡 

分 类 号：R735.1[医药卫生—肿瘤]