RNF8的亚细胞定位及其在胃癌中的表达与凋亡调控  

Subcellular Localization of RNF8 and Its Expression and Role in Apoptosis of Gastric Cancer

作　　者：刘健[1] 雷箴[1] 袁雯 赵枚 黄常志 LIU Jian LEI Zhen YUAN Wen ZHAO Mei HUANG Changzhi(Medical Research Center, Beijing Chaoyang Hospital, Capital Medical University, Beijing 100020, China State Key Laboratory of Molecular Oneology, Beijing Key Laboratory for Carcinogenesis and Cancer Prevention, Department of Etiology and Carcinogenesis, National Cancer Center/Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100021, China)

机构地区：[1]首都医科大学附属北京朝阳医院医学研究中心,北京100020 [2]国家癌症中心/中国医学科学院北京协和医学院肿瘤医院病因室分子肿瘤学国家重点实验室癌发生及预防分子机理北京市重点实验室,北京100021

出　　处：《肿瘤防治研究》2017年第9期575-579,共5页Cancer Research on Prevention and Treatment

基　　金：国家自然科学基金青年基金项目(81502384);中国医学科学院医学与健康科技创新工程(2016-I2M-1-001)

摘　　要：目的研究环指蛋白8(ring finger protein 8,RNF8)的亚细胞定位及其在胃癌中的表达及功能。方法构建RNF8正常及突变的真核表达载体,转染HEK293细胞,激光共聚焦显微镜下观察其亚细胞定位情况;利用Western blot及RT-PCR法分别检测RNF8在胃癌组织及细胞中的表达情况;利用流式细胞仪检测RNF8高表达对胃癌细胞凋亡的影响。结果野生型RNF8主要定位于胞核,其指环(RING)结构域和叉头相关(forkhead-associated domain,FHA)结构域突变均对RNF8的胞核定位有一定的影响;RNF8在胃癌细胞中的表达明显下调;外源导入RNF8可以导致胃癌MGC803细胞发生凋亡。结论 RNF8可能通过调控细胞凋亡参与肿瘤发生发展过程。Objective To investigate the subcellular localization of ring finger protein 8（RNF8） and explore its expression and function in gastric cancer cells.Methods The fusion eukaryotic vectors expressing widetype or mutant EGFP-RNF8 were constructed and transfected into HEK293 cells.The subcellular localization of RNF8 was observed under laser scanning confocal microscope;Western blot and RT-PCR were used to detect RNF8 expression in gastric cancer tissues and cell lines;the role of RNF8 on gastric cancer cell apoptosis was detected using flow cytometry.Results Wild-type RNF8 was mainly located in the nucleus,while both RING and forkhead-associated（FHA） domain mutations could partly affect nuclear localization;RNF8 showed a lower expression pattern in gastric cancer cells and its forced expression in gastric cancer cell line MGC803 led to cell apoptosis.Conclusion RNF8 may regulate the development of gastric tumor through modulating cancer cell apoptosis.

关 键 词：RNF8 亚细胞定位 胃癌 凋亡 

分 类 号：R735.2[医药卫生—肿瘤]