脐橙酸腐菌分离鉴定及抑菌研究  

Identification and antifungal activity research of navel orange Geotrichurn candidum

作　　者：冷飞凡 姜倩 曾慧 李淑惠 淳炯[1,3] 钟八莲 杨文侠[1,3] LENG Fei-fan JIANG Qian ZENG Hui LI Shu-hui CHUN Jiong ZHONG Ba-lian YANG Wen-xia(College of Life and Environmental Sciences, Gannan Normal University, Ganzhou 341000 The Agriculture and Grain Bureau of Yudu County, Ganzhou 342300 National Navel Orange Engineering Research Center, Ganzhou 341000)

机构地区：[1]赣南师范大学生命与环境科学学院,赣州341000 [2]于都县农业和粮食局,赣州342300 [3]国家脐橙工程技术研究中心,赣州341000

出　　处：《食品科技》2017年第9期8-12,共5页Food Science and Technology

基　　金：江西省教育厅科学技术研究项目(14664);江西省科技创新团队建设计划项目(20142BCB24007);江西省科技计划项目(20141BBG70002);赣州市科技计划项目;赣南师范学院科技计划项目(2014QC10)

摘　　要：实验从脐橙病果上分离获得酸腐病病原菌,结合传统手段(病原菌形态学鉴定及柯赫氏法则)和分子方法鉴定为Geotrichurn candidum,并进一步研究了该病原菌生物学特性及抑菌。结果表明:脐橙酸腐菌的最佳生长条件是温度为(25~30)℃,p H为5;抑菌实验表明,农药杀菌剂中,百可得对酸腐菌抑制效果较好,在质量浓度为2 mg/m L时抑菌率达86.65%;植物提取液中川芎的抑菌效果最佳,质量浓度为40 mg/m L时抑菌率达到80.46%,有望开发为新型绿色脐橙防腐剂。A pathogen was isolated from the diseased navel orange and identified as Geotrichurn candidum by traditional methods（morphological identification of pathogens and Koch＇s law） and molecular methods. Its biological characteristics and antifungal activities were further studied. The results showed that the optimal growth conditions of this fungus were as the following： The temperature was（25~30）℃and the p H value was 5. The antifungal experiment showed that Bellkute could inhibit this fungus with the inhibitory rate of 86.65% at the concentration of 2 mg/m L. The inhibitory rate of the extract of Ligusticum Chuanxiong Hort. was 80.46% at the concentration of 40 mg/m L, which may be developed as a novel green preservative for navel orange.

关 键 词：酸腐菌 生物学特性 农药杀菌剂 植物提取液 脐橙 

分 类 号：TS201.3[轻工技术与工程—食品科学]