生黄精多糖与制黄精多糖抑菌效果比较研究  

Compare of antibacterial effect produced by polysaccharides between raw materials and processing Polygonatum sibiricum

作　　者：曹冠华[1] 李泽东[1] 赵荣华[1] 张倩茹[2] 李进波 和志伟 康坤 贺森[1] CAO Guan-hua LI Ze-dong ZHAO Rong-hua ZHANG Qian-ru LI Jin-Bo HE Zhi-wei KANG Kun HE Sen(College of Pharmaceutical Science, Yunnan University of Traditional Chinese Medicine, Kunming 650500 Institute of Pomology, Shanxi Academy of Agricultural Science, Taiyuan 030800 Xishuangbanna State Agricultural Environmental Protection Monitoring Station, Xishuangbanna 666100)

机构地区：[1]云南中医学院中药学院,昆明650500 [2]山西省农业科学院果树研究所,太原030800 [3]西双版纳州农业环境保护监测站,西双版纳666100

出　　处：《食品科技》2017年第9期202-206,共5页Food Science and Technology

基　　金：南药协同创新中心项目(30270101100);国家自然科学基金项目(81560612);云南省科技厅青年项目(2013FD034;2014FD034);云南省教育厅一般项目(2014Y228)

摘　　要：以大肠杆菌、枯草芽孢杆菌、金黄色葡萄球菌为供试菌,比较了生黄精与制黄精多糖的抑菌效果;发现生黄精与制黄精多糖对3株供试菌均有抑制效果,其对大肠杆菌、枯草芽孢杆菌、金黄色葡萄球菌的抑菌圈直径分别为(10.75±0.39)、(10.90±0.94)、(10.20±0.45)mm,(12.55±0.85)、(9.73±0.37)、(11.50±0.27)mm,最小抑菌浓度(MIC)依次为1.23、0.98、1.31 mg/m L,0.67、1.16、0.74 mg/m L。结果显示,制黄精多糖对大肠杆菌、金黄色葡萄球菌的抑制效果较好,生黄精多糖则对枯草芽孢杆菌的抑制作用相对较强。The antibacterial effect produced by polysaccharides was compared in this experiment between raw materials and processing Polygonatum sibiricum with the bacteria of Escherichia coli, Bacillus subtilis and Staphylococcus aureus. The results showed that the strains could be inhibited by Polygonatum polysaccharides extraction. The inhibition zones of raw Polygonatum sibiricum to the above bacteria were（10.75±0.39） mm,（10.90±0.94） mm and（10.20±0.45） mm, while processing Polygonatum sibiricum were（12.55±0.85） mm,（9.73±0.37） mm, and（11.50±0.27） mm. Moreover, corresponding minimal inhibitory concentration（MIC） to the three bacteria were respectively 1.23 mg/m L, 0.98 mg/m L and 1.31 mg/m L for raw Polygonatum polysaccharides and 0.67 mg/m L, 1.16 mg/m L and 0.74 mg/m L for processing Polygonatum polysaccharides. As a result, antibacterial effect produced by processing Polygonatum polysaccharides to Escherichia coli and Staphylococcus aureus was better. Otherwise, raw Polygonatum polysaccharides were easier to inhibit Bacillus subtilis.

关 键 词：黄精多糖 抑菌圈 最小抑菌浓度 大肠杆菌 金黄色葡萄球菌 枯草芽孢杆菌 

分 类 号：R284.2[医药卫生—中药学]