洋葱多肽及蛋白对T2DM小鼠降血糖及降血脂作用研究  

Effect on hypoglycemic and hypolipidemic of onion polypeptide and protein on T2DM mice

作　　者：刘阳[1] 周涵黎 黄光强[1] 吴梦阳 张帅[1] 钱伟亮 吴悠[1] 李丽[1] LIU Yang ZHOU Han-li HUANG Guang-qiang WU Meng-yang ZHANG Shuai QIAN Wei-liang WU You LI Li(Engineering Technology Research Center of Biological Peptide Antidiabetics of Hube Province, Synergy Innovation Center of Biological Polypeptide Antidiabetics of Hubei Province, School of Life Science, Wuchang University of Technology, Wuhan 43022)

机构地区：[1]武昌理工学院生命科学学院生物多肽糖尿病药物湖北省协同创新中心湖北省生物多肽糖尿病药物工程技术研究中心,武汉430223

出　　处：《食品科技》2017年第9期227-232,共6页Food Science and Technology

基　　金：国家级大学生创新训练项目(201612310004);湖北省级大学生创新创业训练计划项目(201512310017);湖北省高等学校优秀中青年科技创新团队计划项目(T201632);武昌理工学院校级重点科研项目(2016K04);武昌理工学院校级科研项目(2015X05)

摘　　要：目的:研究洋葱多肽及蛋白的体内降糖降脂活性。方法:将造模成功的小鼠进行糖耐量实验和药效实验,利用各生化指标参数和动物组织切片,探究洋葱多肽及蛋白的体内降糖及降脂作用。结果:200 mg/kg bw洋葱蛋白及洋葱多肽的降糖效果达到盐酸二甲双胍降糖效果的70%及80%以上;洋葱多肽组则在60、120 min时的血糖值及糖耐量AUC均显著低于模型组,分别达到阳性对照组的87.34%、93.53%和92.44%;洋葱蛋白组的AST和UREA高于正常值但低于模型组,洋葱多肽组与阳性对照组各项指标均在阈值内。结论:洋葱蛋白和洋葱多肽能有效降低2型糖尿病小鼠血糖和血脂,有效提高其口服耐糖量,并能对受损的小鼠肝、肾予以一定程度的恢复,且洋葱多肽的作用优于洋葱蛋白。Objective： To investigate the hypoglycemic and hypolipidemic activity of the onion polypeptide and protein in vivo. Methods： A model of T2 DM mice was established by feeding with high glucose and fat diet combined with streptozocin. Glucose tolerance test and drug efficacy test were carried out on the T2 DM model mice. In addition, the biochemical indexes and the animal tissue section were utilized to explore the hypoglycemic and hypolipidemic effect of onion polypeptide and protein in vivo. Results： The hypoglycemic activity of onion protein（200 mg/kg bw） and onion polypeptide（200 mg/kg bw） reached more than 70% and 80% of metformin（400 mg/kg bw）. Furthermore, the blood glucose level at 60, 120 min, and glucose tolerance of AUC of onion polypeptide group were significantly lower than that of the model group, decreased by 27.72%, 38.80% and 25.99% than that of the model group, respectively, and reached to 87.34%, 93.53% and 92.44% that of the normal group, respectively. Moreover, the level of AST and UREA of onion protein group were higher than that of the normal group, but lower than that of model group, and all biochemical indexes of onion polypeptide group and positive control group were within the threshold. Conclusion： Onion polypeptide and protein could reduce the level of blood glucose and lipid, improve the oral glucose tolerance of T2 DM mice, and recover the heart, liver and kidney damage in T2 DM mice, and the hypoglycemic and hypolipidemic effect of onion polypeptide was better than onion protein.

关 键 词：洋葱多肽 洋葱蛋白 T2DM小鼠 降糖降脂 

分 类 号：R284.2[医药卫生—中药学]