大豆及豆制食品中转基因成分检测分析  

Detection and analysis of genetically modified organisms in soybean and soybean products

作　　者：王超[1] 谭冬梅[1] 王红[1] 瞿聪 韦程媛[1] WANG Chao TAN Dong-mei WANG Hong QU Cong WEI Cheng-yuan(Guangxi Zhuang Autonomous Region Center for Disease Control and Prevention, Nanning 53002)

机构地区：[1]广西壮族自治区疾病预防控制中心,南宁530028

出　　处：《食品科技》2017年第9期315-321,共7页Food Science and Technology

基　　金：广西壮族自治区疾病预防控制中心青年基金项目(GXCDC201505)

摘　　要：建立大豆及豆制食品中转基因成分分析的real-time PCR扩增体系和方法。选择市售的大豆、豆粉、豆干、豆腐、豆浆和腐竹等6种大豆及豆制食品,针对不同的样品探索高质量基因组DNA的提取方法,扩增大豆内参基因lectin进行质量检测。针对我国批准的3种主要转基因大豆品系(GTS40-3-2,A2704-12和MON89788)设计特异性的引物和探针,进行real-time PCR检测。购买3种转基因大豆品系的标准品作为质控对照。从该6种大豆及豆制食品中均提取到了高质量的基因组DNA,建立了稳定的针对外源基因特异性、结构特异性和品系特异性片段检测的real-time PCR扩增体系。成功建立了6种大豆及豆制食品中针对3种转基因大豆品系成分检测的real-time PCR方法。To build the real-time PCR system and methods for analysis of genetically modified organisms（GMOs） in soybean and soybean products. Choose six commercially available soybeans and soybean products including soybean, soybean powder, dried tofu, tofu, soybean milk and dried beancurd sticks and explore the methods of extracting genomic DNA with high quality from different samples. Amplify soybean reference gene of lectin to process quality test. Design specific primers and probes for the three main transgene soybean events approved in China（GTS 40-3-2, A2704-12 and MON89788） and test with real-time PCR. Process the quality control by purchasing the certified reference material of these three transgene soybean events. The high quality genomic DNA had been extracted from all these six soybean and soybean products and the stable real-time PCR amplification system had been built for these six soybean and soybean products against exogenous gene specific, construct specific and event specific fragments. The real-time PCR methods have been built successfully targeting three transgene soybean events in six soybean and soybean products.

关 键 词：大豆制品 转基因成分检测 转基因大豆品系 GTS40-3-2 A2704-12 MON89788 REAL-TIME PCR 

分 类 号：TS214.2[轻工技术与工程—粮食、油脂及植物蛋白工程]