益气化瘀解毒方对慢性萎缩性胃炎癌前病变的疗效及对细胞周期蛋白E表达和肿瘤标记物的影响  

Therapeutic Effect of Supplementing Qi and Removing Blood Stasis and Removing Toxin on Precancerous Lesions of Chronic Atrophic Gastritis and Its Effect on Cell Cycle Protein E Expression and Tumor Marker

作　　者：付利然[1] 张士金[1] 郭伟胜 

机构地区：[1]郑州人民医院中医科,河南郑州450000 [2]河南中医药大学第二附属医院肿瘤外科,河南郑州450002

出　　处：《中华中医药学刊》2017年第10期2718-2720,共3页Chinese Archives of Traditional Chinese Medicine

摘　　要：目的:探讨益气化瘀解毒方对慢性萎缩性胃炎癌前病变的疗效及对细胞周期蛋白E表达和肿瘤标记物的影响。方法:选择2015年1月—2016年6月在医院接受治疗的90例气虚血瘀型萎缩性胃炎癌前病变患者为研究对象,按照随机数字表法分为对照组(45例)和观察组(45例),对照组给以常规治疗,观察组在对照组基础上给予口服益气化瘀解毒方,3个疗程后观察临床疗效,胃痛、纳差、乏力、嗳气、便溏中医证候积分,免疫组化法检测细胞周期蛋白E(Cyclin E)、E-钙黏着蛋白(E-cadherin)和基质金属蛋白酶9(MMP-9)蛋白表达,ELISA法检测血清肿瘤标志物白介素-2(IL-2)、白介素-10(IL-10)、干扰素-γ(IFN-γ)和肿瘤坏死因子-α(TNF-α)水平。结果:治疗3个疗程后,观察组总有效率93.33%高于对照组的75.56%(χ~2=5.414,P=0.020)。观察组的胃痛、纳差、乏力、嗳气、便溏中医证候积分均低于对照组(P<0.05)。观察组患者的胃组织Cyclin E和MMP-9蛋白表达低于对照组,而E-cadherin蛋白表达高于对照组(P<0.05)。观察组患者血清肿瘤标志物IL-2、IL-10和TNF-α高于对照组,而IFN-γ低于对照组(P<0.05)。结论:益气化瘀解毒方对慢性萎缩性胃炎癌前病变具有较好的临床疗效,可降低中医证候积分,降低细胞周期蛋白E表达,改善患者病情进展。Objective： To explore the therapeutic effect of supplementing Qi and removing blood stasis and toxin on precancerous lesions of chronic atrophic gastritis and its effect on the expression of cell cycle protein E and tumor markers. Methods ： From January 2015 to June 2016 in our hospital,90 cases of Qi deficiency and blood stasis patients with atrophic gastritis precancerous lesions were as the research objects, and randomly divided into the control group （45 cases） and the observation group （45 cases）. The control group were given routine treatment and the observation group on the basis of the control group were given orally Yiqihuayu Decoction and clinical effect, stomach pain, anorexia, fatigue, belching, loose stools, TCM syndrome score and immunohistochemical method was observed after 3 courses. Detect cell cycle protein E （ Cyclin E） , E - cadherin （ E - cadherin） and matrix metalloproteinases 9 （ MMP - 9） protein expression and serum tumor ELISA method markers of interleukin - 2 （ IL - 2） , interleukin - 10 （ IL - 10） and interferon gamma （IFN -gamma） and tumor necrosis factor alpha （TNF -alpha） levels. Results： After 3 courses of treatment, the total effective rate of the observation group was 75.56%, higher than that of the control group＇ s 93.33% （ Х^2 = 5. 414, P = 0. 020）. The observation group＇ s pain, anorexia, fatigue, belching, loose stools and TCM syndrome integral were lower than those of the control group （ P 〈 0. 05 ）. In the observation group, the expression of E Cyclin and MMP - 9 protein in gastric tissue were lower than those in control group while the expression of E - cadherin protein was higher than that of control group （P 〈 0. 05）. The serum tumor markers IL - 2, IL - 10 and TNF - α in the observation group were higher than those in the control group while the IFN - γ was lower than those of the control group （ P 〈 0. 05 ）. Conclusion ： The method of supplementing Qi and removing blood stasis toxin in treating pre

关 键 词：益气化瘀解毒方 慢性萎缩性胃炎 胃癌前病变 细胞周期蛋白E 肿瘤标志物 

分 类 号：R735.2[医药卫生—肿瘤]