体检人群小麦不耐受阳性率及其组分的分析研究  

Analysis and study on wheat intolerance positive rate and its components in physical examination population

作　　者：戈立秀 张丽[2] 张伟[1] 韩煦[1] 常艳敏[1] 

机构地区：[1]天津市南开医院检验科,300100 [2]天津医科大学检验学院,天津300203

出　　处：《检验医学与临床》2017年第19期2827-2829,共3页Laboratory Medicine and Clinic

基　　金：天津市卫生和计划生育委员会科技基金资助项目(2010Ky12)

摘　　要：目的分析体检人群小麦免疫球蛋白G(IgG)抗体水平,探讨体检人群小麦不耐受的阳性率与性别、年龄间的关系,分析其不耐受组分。方法采用乙醇-丙酮法对小麦蛋白进行提取,用粗提小麦蛋白包被96微孔板,酶联免疫吸附试验检测122例体检者小麦蛋白IgG水平,SDS-PAGE电泳分析其蛋白组分,并用酶免疫印迹方法分析提取液中与IgG特异度结合的主要蛋白组分。结果该地区体检人群小麦不耐受抗体阳性率为21.3%,其中男(18.52%)、女(23.53%)阳性率差异无统计学意义(P>0.05);少年组阳性率为26.47%,中年组17.24%,老年组为18.64%,其中少年组与中年组、老年组的差异均有统计学意义(P<0.05),而老年组和中间组之间差异无统计学意义(P>0.05);酶免疫印迹结果显示,小麦蛋白不耐受组分主要有4种,主要为麦谷蛋白,且个体间所针对的抗原差异无统计学意义(P>0.05)。结论体检人群中部分存在小麦蛋白IgG抗体;且小麦蛋白不耐受与性别无相关性,而与年龄有一定相关性;不同个体间小麦主要不耐受组分差异无相关性。Objective To analyze the wheat-specific IgG level in physical examination population,and to investigate the relation between the wheat intolerance of physical examination population with the age and gender,and to analyze its intolerance components.Methods The wheat protein was extracted by using ethanol and acetone extraction method,and the 96 microplates were coated with the crudely extracted wheat protein.The wheat-specific IgG level was detected by using the enzyme linked immunosorbent assay（ELISA）in 122 persons undergoing physical examination.The components of crude protein were analyzed by SDS-PAGE electrophoresis and the main protein ingredients in the extraction solution were identified by Western-blotting.Results The positive rate of wheat intolerance antibody among the physical examination population in local area was 21.3%,in which the positive rate was 23.53%for females and 18.52%for males,but the difference was not statistically significant（P〉0.05）;the positive rate was26.47%in the teenager group,17.24%in the middle-aged group,18.64%in the elderly group,the difference between the teenager group with middle-age group and elderly group were statistically significant（P〈0.05）,while the difference between the elderly group and middle-aged group was not statistically significant（P〉0.05）;Western-blot results showed that there were four main intolerance components in the wheat,in which glutenin was predominant,moreover the antigen difference among different individuals had no statistical significance（P〉0.05）.Conclusion The wheat-specific IgG antibodies exist in partial physical examination population;moreover the wheat protein intolerance has no correlation with gender and has a certain correlation with the age;the difference in main components of wheat intolerance among different individuals has no statistical significance（P〉0.05）.

关 键 词：食物不耐受 小麦蛋白 免疫球蛋白G 体检人群 

分 类 号：R965[医药卫生—药理学]