芽孢杆菌ZJM-P5与氮配施对红小豆幼苗生长和氮吸收的影响  

Effects of Bacillus ZJM-P5 and Nitrogen Application on Growth and Nitrogen Uptake of Adzuki Bean Seedlings

作　　者：栾换换 裴红宾[1] 张永清[1,2] 高振峰 

机构地区：[1]山西师范大学生命科学学院,山西临汾041004 [2]山西师范大学地理科学学院,山西临汾041004 [3]山西农业大学农学院,山西太谷030801

出　　处：《西北植物学报》2017年第8期1550-1558,共9页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31571604);山西师范大学科技开发与应用基金(YK1502)

摘　　要：以红小豆品种‘晋红小豆5号’和促生芽孢杆菌ZJM-P5为材料,设置不同氮素用量和菌液浓度处理,采用盆栽试验,测定各菌氮处理下红小豆幼苗农艺性状、生理特性及含氮量等指标,研究促生芽孢杆菌ZJM-P5与氮配施对红小豆幼苗生长发育的影响,并筛选出最佳促生菌液浓度水平,为提高红小豆幼苗的养分吸收利用效率及其高产优质栽培提供理论依据。结果表明:(1)在同一菌液浓度下,红小豆苗期株高、茎粗、叶面积、地上部干重、根系干重、根表面积、根系体积、根系平均直径均随施氮量的增加呈先升高后降低的趋势,而最大根长随着施氮量增加有所下降;根系可溶性糖及MDA含量随施氮量增加均呈先降后升的趋势;根系SOD、POD活性及根系活力均随施氮量增加先升高后降低;根系可溶性蛋白含量、NR活性和植株含氮量均随施氮量增加而增加。(2)在相同施氮量条件下,除根系可溶性糖和MDA含量随着菌浓度的增加先下降后升高外,其余各指标均呈先升后降的变化趋势;各指标除低氮(50mg·kg^(-1))水平下在10~8 cfu·mL^(-1)菌液浓度达到最大值外,其它氮水平均在10~7 cfu·mL^(-1)菌液浓度达到最大值。研究发现,芽孢杆菌ZJM-P5与氮合理配施有利于红小豆植株的氮素吸收和生长发育,并以100mg·kg^(-1)为最佳施氮(纯氮)水平,10~7 cfu·mL^(-1)为最佳菌浓度,且两者组合处理的幼苗农艺性状、根系活力及保护酶活性、氮素含量均达到较高水平;过量施氮、施菌或菌氮比例不合理则会导致幼苗干物质积累及氮素提升受限,进而抑制植株正常生长。We took the adzuki bean cultivar ‘Jin adzuki bean 5' and growth promoting bacteria Bacillus ZJM-P5 as materials,set different nitrogen dosage and concentration of bacteria treatments, and determined agronomic characters,physiological characteristics and nitrogen content under different treatments by pot experiments at seedling stage.The effect of different Bacillus ZJM-P5 and nitrogen treatments ongrowth and development of adzuki bean seedlings was analyzed, and the optimum growth promoting bacteria concentration level was screened.The aim is to improve the seedling quality and nutrient absorption and utilization efficiency of adzuki bean, and provide a theoretical basis for high yield and high quality cultivation.The results showed that:(1)under the same concentration of bacteria fluid,plant height,stem diameter,leaf area,shoot dry weight,root dry weight,root area,root volume and average root diameter initially increased and then decreased with increasing nitrogen application rate;However the max root length showed a decline;Furthermore,the contents of soluble sugar and MDA in the roots decreased firstly and then increased with the increase of nitrogen application rate,while the activities of SOD,POD in root and root activities initially increased and then decreased with increasing nitrogen rate;the soluble protein content in the root,NR activity and nitrogen uptake of plant rose with increasing nitrogen rate.(2)Under the equal amount of nitrogen fertilizer,in addition to soluble sugar and MDA contents in roots with the increase of bacteria concentration firstly decreased and then increased.The other indexes showed a trend of increasing firstly and then decreasing;All the indexes in addition to the low nitrogen level(50mg·kg^(-1)),the concentration of 10~8 cfu·mL^(-1) bacteria solution reached the maximum value, and the other nitrogen levels reached the highest value at the concentration of 10~7 cfu·mL^(-1) bacteria solution.The study found that reasonable combined application of Bacillus ZJM-P5 and nitroge

关 键 词：红小豆 氮 芽孢杆菌ZJM-P5 促生 氮素吸收 

分 类 号：Q945.78[生物学—植物学]