DMBT1过表达对鼻咽癌细胞生物学行为的影响  

Effects of overexpression of DMBT1 on biological behavior of nasopharyngeal carcinoma cells in vitro

作　　者：刘新彦[1] 李建斌[2] 李志强[3] 王丽坤[1] 路继成 李晓丽[1] 刘晓燕[1] 

机构地区：[1]河北北方学院附属第二医院耳鼻咽喉头颈外科,河北省张家口市075100 [2]中国石油天然气集团公司中心医院耳鼻咽喉科 [3]河北北方学院附属第二医院病理科,河北省张家口市075100 [4]河北省张家口市宣化区人民医院检验科

出　　处：《河北医药》2017年第20期3051-3054,共4页Hebei Medical Journal

基　　金：河北省医学科学研究重点课题(编号:20170181)

摘　　要：目的观察DMBT1过表达对鼻咽癌细胞生物学行为的影响。方法利用脂质体Lipofectamine TM 2000转染鼻咽癌5-8F细胞,Real time-PCR、Western-blot验证转染效率。MTT、流式细胞术检测DMBT1过表达对5-8F细胞增殖、凋亡和细胞周期的影响。Western-blot检测DMBT1过表达对多药耐药基因(P-gp)、肿瘤抑制基因p53蛋白表达的影响。MTT法检测DMBT1过表达对化疗药物敏感性的影响。结果转染pc DNA3.1/DMBT1的5-8F细胞中DMBT1表达显著上调(P<0.05),提示成功构建稳定表达DMBT1基因的鼻咽癌5-8F细胞。DMBT1过表达的鼻咽癌5-8F细胞增殖能力减慢、凋亡率、G0/G1期细胞比例及p53蛋白表达增高,S期、G2/M期细胞比例及P-gp蛋白表达降低,同时细胞对顺铂敏感性增加(P<0.05)。结论 DMBT1过表达能够显著抑制鼻咽癌5-8F细胞增殖,诱导其凋亡,并提高癌细胞对化疗药物敏感性。Objective To observe the effects of overexpression of DMBT1 on biological behavior of nasopharyngeal carcinoma cells （NPC） in vitro. Methods The NPC cell line-5-8F cells were transfected by lipofectamine TM 2000,then the expression levels of DMBT1 were detected by Real time-PCR and Western Blot. The effects of DMBT1 overexpression on proliferation,apoptosis and cell cycle were detected by MTT and flow cytometry. Moreover the effects of DMBT1 overexpression on the expressions of P-gp and p53 were detected by Western Blot. Moreover the effects of DMBT1 overexpression on susceptibility of chemotherapeutics were detected by MTT. Results The expression levels of DMBT1 in 5-8F cells transfected by pcDNA3. 1/DMBT1 were significantly up-regulated （P〈0. 05）,which suggested that the 5-8F cells that could express stably DMBT1 gene were successfully established. The overexpression of DMBT1 inhibited the proliferation and promoted the apoptosis of 5-8F cells obviously,and the proportion of cells at G0/G1 stage and the expression of p53 protein were increased significantly,however, the proportion of cells at stage S and G2/M and the expression of P-gp protein were decreased significantly,meanwhile the sensitivity of tumor cells to cisplatin was obviously increased by overexpression of DMBT1 （ P〈0. 05）. Conclusion The overexpression of DMBT1 can significantly inhibit the cell proliferation of NPC,induce its apoptosis and can enhance the sensitivity of cancer cells to chemotherapeutics.

关 键 词：DMBT1 鼻咽癌 基因功能 生物学行为 

分 类 号：R739.6[医药卫生—肿瘤]