靶基因iNOS、EPO表达对卵巢上皮性肿瘤发生发展的影响机制研究  

Study on the influencing mechanism of target genes iNOS and EPO expression on the occurrence and development of ovarian epithelial tumors

作　　者：苏雪锋[1] 陈桂丽 邹云峰[2] 

机构地区：[1]浙江省余姚市人民医院妇产科,浙江余姚315400 [2]浙江省余姚市人民医院病理科,浙江余姚315400

出　　处：《中国现代医生》2017年第25期22-25,共4页China Modern Doctor

基　　金：浙江省宁波市自然科学基金项目(2016A610026)

摘　　要：目的研究靶基因诱导型一氧化氮合酶(iNOS)、促红细胞生成素(EPO)在卵巢上皮性肿瘤中的表达对卵巢上皮性肿瘤发生发展的影响机制。方法随机选取2014年3月~2016年12月我院手术切除并经病理证实的卵巢上皮性肿瘤组织和正常卵巢组织标本共84例。患者均由手术切除取标本切片,经甲醛固定,石蜡包埋,作4μm连续切片,分别进行HE染色及免疫组化染色。综合染色强度和阳性细胞占总细胞数的百分比进行半定量处理,计数肿瘤细胞总数和阳性细胞个数,计算阳性百分率。结果正常卵巢组织上皮细胞中i NOS和EPO几乎没有表达,从良性卵巢肿瘤-卵巢交界性肿瘤-恶性卵巢肿瘤,iNOS表达阳性率分别为17.39%、47.06%、87.50%,EPO表达阳性率分别为73.91%、88.24%、100.00%,差异有统计学意义(P<0.05)。肿瘤的组织学分级与EPO及i NOS的表达有关,肿瘤分化程度越低,EPO及iNOS的表达含量越高,且EPO与患者的临床分期有关,差异有统计学意义(P<0.05);而iNOS与患者的临床分期无关(P>0.05)。分析发现,恶性卵巢上皮性肿瘤中EPO蛋白表达和iNOS蛋白表达之间呈正相关,差异具有统计学意义(P<0.05)。结论 EPO蛋白和iNOS蛋白在卵巢上皮性肿瘤能促进肿瘤细胞的增殖及血管生成,对卵巢上皮肿瘤的发生、发展具有一定的预测作用。Objective To study the influencing mechanism of expression of target gene-inducing nitric oxide synthase(iNOS) and erythropoietin(EPO) in ovarian epithelial tumors on the occurrence and development of ovarian epithelial tumors. Methods A total of 84 cases of specimens of ovarian epithelial tumor tissue and normal ovarian tissue who were given incision and pathologically confirmed in our hospital from March 2014 to December 2016 were randomly selected. Patients were all given surgical incision to remove the slices of specimens, fixed by formaldehyde, embedded by paraffin to make a 4 μm continuous slices. Immunohistochemical staining and HE staining were performed respectively.Comprehensive staining intensity and percentage of positive cells in the total number of cells were used to perform se-mi-quantitative treatment. The total number of tumor cells and the number of positive cells were counted, and the positive percentage was calculated. Results iNOS and EPO in normal oviran epithelial cells were barely expressed. The positive rates of iNOS expression were 17.39%, 47.06% and 87.50% respectively in benign ovarian tumors-ovarian borderline tumors-malignant ovarian tumors. The positive rates of EPO expression were 73.91%, 88.24% and 100.00%respectively. The differences were statistically significant(P<0.05). The histological grade of tumor was related to the expression of EPO and iNOS.The lower the degree of tumor differentiation, the higher the expression level of EPO and iNOS. EPO was correlated with the clinical stage of the patients, and the difference was statistically significant(P<0.05);and iNOS was not correlated with clinical stage(P>0.05). There was a positive correlation between EPO protein expression and iNOS protein expression in malignant epithelial ovarian tumors, and the difference was statistically significant(P<0.05).Conclusion EPO protein and i NOS protein can promote tumor cell proliferation and angiogenesis in epithelial ovarian tumors, and have a certain predictive effect on the occurrence an

关 键 词：靶基因iNOS EPO 卵巢上皮性肿瘤 影响机制 

分 类 号：R737.3[医药卫生—肿瘤]