Acvr1敲除对牙本质形成的影响研究  

The role of BMP type I receptor ACVR1 in regulating dentin formation

作　　者：张雪 刘麒麟[3] 李媛[1,2] 赵欢 刘苍维[1,2] 闫广兴 胡月 李道伟 史册 孙宏晨[1,2] 

机构地区：[1]吉林省牙发育及颌骨重塑与再生重点实验室,吉林长春130021 [2]吉林大学口腔医院口腔病理科,吉林长春130021 [3]吉林大学口腔医院口腔颌面外科,吉林长春130021

出　　处：《口腔生物医学》2017年第3期132-135,共4页Oral Biomedicine

基　　金：国家重点研发项目(2016YFC1102804);国家自然科学基金国际(地区)合作与交流项目(81320108011);国家自然科学基金青年基金项目(81600843;81600823;81600890);中国博士后科学基金(2017M611332);吉林省卫生厅项目(2016Q025);吉林省科技计划项目(20170520016JH;20170520009JH)

摘　　要：目的:探讨牙乳头细胞中活化蛋白A受体1基因(ACVR1)敲除对牙本质形成的影响。方法:以同窝Osterix-Cre;Acvr1fx/-基因型小鼠为实验组,Osterix-Cre;Acvr1fx/+小鼠为对照组,利用影像学方法和HE染色初步观察Acvr1条件性基因敲除对小鼠牙本质形成的影响;免疫组织化学染色检测成牙/成骨分化相关蛋白水平;实时定量RT-PCR技术检测成牙/成骨分化相关因子的表达。结果:micro-CT显示,实验组下颌骨表现为疏松多孔样结构,磨牙牙本质变薄,前期牙本质增厚,HE染色显示成牙本质细胞排列紊乱,而切牙出现骨样牙本质;实验组磨牙成牙本质细胞分化相关基因Dspp、Nestin表达有下调趋势(无统计学差异),切牙Dspp表达有下调趋势(无统计学差异),免疫组织化学染色显示实验组磨牙DSPP表达减少。结论:ACVR1通过调控成牙本质细胞分化而影响牙本质形成,在调控成牙本质细胞成牙转化过程中具有重要作用。Objective： To explore the effect of knockout of Acvr1（ activin A receptor type 1） on the formation of dentin. Methods：Acvr1 conditional knockout mice model was constructed using Cre-lox P system. The genotype Osterix-Cre; Acvr1fx/-was chosen as the experiment group and the genotype Osterix-Cre; Acvr1fx/＋was chosen as the control group in littermate. We observed the dentin formation by imageology and HE staining. And we checked the expression of the odontoblast/osteoblast markers by immunohistochemical staining. Finally the expression of odontoblast/osteoblast genes was detected by qRT-PCR. Results： The mandibles of the experiment group showed more pores,the dentin of mandibular first molar was thinner,pre-dentin was thicker,and odontoblast looked irregular. The genes important for odontoblast differentiation such as Dspp,Nestin were downregulated. However,the incisor showed osteodentin,and the Dspp was down-regulated. IHC showed down-regulated DSPP in c KO molar. Conclusions： ACVR1 affected the formation of dentin by up-regulating odontoblast differentiation,and also had important role in odontoblast transition.

关 键 词：成牙本质细胞 活化蛋白A受体 牙本质 

分 类 号：R780.2[医药卫生—口腔医学]