肿瘤靶向的磁性荧光IR780-Fe_3O_4纳米颗粒用于循环肿瘤细胞的检测  

Tumor Targeting of Fluorescent Magnetic IR780-Fe_3O_4 Nanoparticles for Detection of Circulating Tumor Cells

作　　者：孙枝红 周理华 邓冠军 郑明彬[1,2,3] 言伟强 李文军[1] 蔡林涛[1] 龚萍[1,2,3] 

机构地区：[1]中国科学院深圳先进技术研究院生物医药与技术研究所纳米特殊制剂工程实验室,深圳518055 [2]广东医科大学广东天然药物研究与开发重点实验室,东莞523808 [3]湖南大学化学生物传感与计量学国家重点实验室,长沙410081 [4]北京大学深圳医院放射科,深圳518036

出　　处：《分析化学》2017年第10期1427-1433,共7页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(Nos.21375141;81671758);深圳市专项资金(Nos.JSGG20160331185422390;JCYJ20150403091443298;JCYJ20150521094519473;JCYJ20160429191503002)资助~~

摘　　要：循环肿瘤细胞(Circulating tumor cells,CTCs)的简单、快速分离和检测是目前临床研究中面临的一项挑战。本研究制备了具有肿瘤靶向识别作用的磁性荧光IR780-Fe_3O_4纳米颗粒,并将其用于CTCs的分离和检测。通过电镜、荧光光谱仪和超导量子干涉仪对合成的IR780-Fe_3O_4纳米颗粒进行表征;采用激光共聚焦显微镜和流式细胞仪对IR780-Fe_3O_4纳米颗粒对肿瘤细胞和正常细胞的靶向效果进行了分析;利用激光共聚焦显微镜对IR780-Fe_3O_4纳米颗粒在MCF-7细胞中的位置进行定位;并根据IR780-Fe_3O_4纳米颗粒孵育后肿瘤细胞的荧光强度绘制标准曲线。研究结果表明,IR780-Fe_3O_4能很好地靶向多种CTCs。细胞定位实验进一步表明,IR780-Fe_3O_4主要靶向识别肿瘤细胞的线粒体。通过Fe_3O_4磁性纳米颗粒偶联IR780建立的这种方法可很好地区分肿瘤细胞和正常细胞,并对模拟血液中的CTCs进行了分离和检测。The tumor targeted fluorescent magnetic IR780-Fe_3O_4 nanoparticles were prepared for separation and detection of circulating tumor cells（CTCs）.These IR780-Fe_3O_4 nanoparticles were characterized by electron microscopy,fluorescence spectrometer,and superconducting quantum interferometer.The targeting effect of IR780-Fe_3O_4 nanoparticles was analyzed on the tumor and normal cells by confocal microscope and flow cytometry,and the confocal microscope was used to target the location of IR780-Fe_3O_4 nanoparticles in MCF-7 cells.The standard curve was drawn and evaluated accorded to the IR780-Fe_3O_4 nanoparticles fluorescence intensity of tumor cells after incubation.The results showed that IR780-Fe_3O_4 nanoparticles could target a variety of CTCs.Furthermore,cellular localization experiment proved that IR780-Fe_3O_4 nanoparticles could target the mitochondria of tumor cells.With the method of coupling magnetic Fe_3O_4 nanoparticles,IR780 could well distinguish the tumor and normal cells,which could be used for separating and detecting the CTCs in simulated blood.

关 键 词：循环肿瘤细胞 荧光强度 IR780 Fe3O4磁性纳米颗粒 

分 类 号：O657.3[理学—分析化学] R730.4[理学—化学] TB383.1[医药卫生—肿瘤]