姜黄素对阿尔茨海默病模型小鼠脑内胶质细胞介导的炎症反应的抑制作用  

Inhibitory effect of curcumin on glial cell-mediated inflammation in a mouse model of Alzheimer's disease

作　　者：方力群[1] 王亚楠 高红梅 陈左翼[1] 叶丹红 

机构地区：[1]哈尔滨医科大学附属第四医院,黑龙江省哈尔滨市150001 [2]黑龙江省护理高等专科学校,黑龙江省哈尔滨市150036

出　　处：《国际神经病学神经外科学杂志》2017年第4期347-351,共5页Journal of International Neurology and Neurosurgery

基　　金：哈尔滨市科技创新人才研究专项资金(2013RFQYJ079);黑龙江省教育厅(12531307)

摘　　要：目的探讨姜黄素对AD模型小鼠学习记忆能力的影响。方法将80小鼠按随机原则分四组,对照组、Alcl3组、溶剂组及姜黄素组。Alcl3制作小鼠AD模型,之后给予腹腔注射姜黄素3 d,给药后14 d水迷宫测试学习记忆能力。免疫染色及western blot方法检测Aβ42、GFAP和Iba-1β蛋白水平。ELISA方法检测海马区炎症因子IL-1β,IL-6和TNF-α的水平。结果与对照组比,Alcl3能够导致逃避潜伏期延长和记忆下降更明显,说明AD模型成功建立。与Alcl3组和溶剂治疗组相比,姜黄素组明显减少潜伏期及增加穿越次数,而且显著减少Aβ42表达。相比Alcl3组,姜黄素组的GFAP和Iba-1β以及海马区IL-1β、IL-6及TNF-α的指标下降有显著意义。结论研究表明姜黄素有潜在治疗AD的可能,通过下调Alcl致胶质细胞过度活化引起炎症反应,而提高学习记忆能力。Objective To investigate the effect of curcumin on the learning and memory ability of a mouse model of Alzheimer＇s disease （AD）.Methods Eighty mice were randomly divided into control,aluminum chloride （AlCl3）,solvent,and curcumin groups.AlCl3-induced AD mice were treated by intraperitoneal injection of curcumin for 3 days.At 14 days after curcumin treatment,the effect of curcumin on learning and memory ability was evaluated using Morris water maze test,and curcumin was also assessed for its effects on the expression levels of amyloid protein42 （Aβ42）,glial fibrillary acidic protein （GFAP）,and ionized calcium binding adapter molecule-1 beta （Iba-1β） by immunostaining and Western blot.The expression levels of the inflammatory cytokines interleukin-1 beta （IL-1β）,IL-6,and tumor necrosis factor-alpha （TNF-oα） were measured by enzyme-linked immunosorbent assay at the same time.Results Compared with the control group,the treatment with AlCl3 significantly prolonged escape latency and memory impairment,indicating the successful establishment of AD mice model.The curcumin group had significantly decreased escape latency,significantly increased passing times,and significantly reduced Aβ42 production,as compared with the AlCl3 and solvent groups.The expression levels of GFAP,Iba-1β,and inflammatory cytokines IL-1β,IL-6,and TNF-oα in the hippocampus in the curcumin group decreased significantly compared with those in the AlCl3 group.Conclusions These findings suggest that curcumin may be a potential treatment of AD,which can improve the learning and memory fumction through reducing inflammatory response caused by AlCl3-induced glial cell activation.

关 键 词：阿尔茨海默病 姜黄素 胶质细胞 炎症反应 小鼠 

分 类 号：R749.16[医药卫生—神经病学与精神病学]