超声波辅助提取蒜苔中总黄酮工艺的响应面优化  

Optimization of ultrasonic-assisted extraction of total flavonoids from garlic sprout using response surface methodology

作　　者：郑梅霞[1] 朱育菁[1] 刘波[1] 陈峥[1] 黄素芳[1] 阮传清[1] 刘欣[1] ZHENG Mei-xia ZHU Yu-jing LIU Bo CHEN Zheng HUANG Su-fang RUAN Chun-qing LIU Xin(Fujian Academy of Agricultural Sciences, Agricultural BioResources Institute, Fuzhou 35000)

机构地区：[1]福建省农业科学院农业生物资源研究所,福州350003

出　　处：《中国食品添加剂》2017年第9期61-67,共7页China Food Additives

基　　金：国家自然科学基金(31370059);福建省公益类科研院所专项(2015R1018-2);福建省农业科学院科技创新项目(2015CX-7)

摘　　要：对提取蒜台中总黄酮的工艺条件进行响应面优化,旨在获得更高的提取率。在单因素试验的基础上,进行超声波辅助提取蒜台中总黄酮工艺的响应面优化,以乙醇浓度(%)、料液比(m L·g^(-1))、超声温度(℃)和超声时间(min)四个参数为自变量,以总黄酮的提取率为响应值,采用Box-Behnken试验设计方法,研究各自变量及其相互作用对总黄酮提取率的影响。超声波辅助提取蒜台中总黄酮的响应面优化得到最佳提取工艺条件为:81%的乙醇浓度、51 m L·g^(-1)的料液比、61℃的超声温度、105 min的超声时间,此条件下蒜苔总黄酮提取率为0.6799%,影响提取的因素为:乙醇浓度>超声时间>料液比>超声温度。响应面方法建立的模型有效,超声波辅助提高了提取率,为蒜台中总黄酮的提取提供参考。Optimizing the processing conditions for extracting total flavonoids from garlic sprout using response surface methodology to improve the extraction yield was studied. Based on the single-factor experiments and Box- Benhnken design, the effect of four factors on the yield of total flavonoids was investigated. Factors including ultrasonic powder, ethanol concentration, liquid-material ratio, ultrasonic temperature and time and their interactions on the yield of extraction were studied using response surface analysis. The optimal extraction conditions of total flavonoids from garlic sprout were： ethanol concentration 81%, liquid-material ratio 51 mL ~ gq, ultrasonic temperature 61 ~C and ultrasonic time 105 rain. The yield of total flavonoids could reach to 0.6799% under the optimal extraction condition. The descending order of influence factors is ethanol concentration, ultrasonic time, liquid- material ratio, and ultrasonic temperature. The regression model was effective and ultrasonic-assisted extraction increased the yields. The results could provide a reference for the total flavonoids extraction from garlic sprout.

关 键 词：蒜苔 总黄酮 超声波 响应面 优化 

分 类 号：O426[理学—声学]