白花蛇舌草总黄酮提取工艺及其抗氧化活性研究  

Study on Extraction and Antioxidant Activities in Vitro of Total Flavonoids from Hedyotis diffusa Willd

作　　者：张俊霞 王应玲 姜宁 罗兴武 黄洋 向极钎 刘晓鹏 ZHANG Jun-xia WANG Ying-ling JIANG Ning LUO Xing-wu HUANG Yang XIANG Ji-qian LIU Xiao-peng(School of Biological Science and Technology, Hubei Institute for Nationalities, Enshi 445000, Hubei, China No.2 Geological Brigade of Geological Department of Hubei Province,Enshi 445000, Hubei, China Enshi QingJiang Bio-Engineering Co., Ltd., Enshi 445000, Hubei, China)

机构地区：[1]湖北民族学院生物科学与技术学院,湖北恩施445000 [2]湖北省地质局第二地质大队,湖北恩施445000 [3]恩施清江生物工程有限公司,湖北恩施445000

出　　处：《湖北农业科学》2017年第18期3523-3527,共5页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(81460573);国家"十二五"科技支撑计划项目(2011BAI04B04-5);江苏省"企业博士后集聚计划"项目(2011033);2013年湖北省战略性新兴(支柱)产业人才培养项目(2013029);2014年度湖北省本科高校"专业综合改革"试点项目(2014029)

摘　　要：研究白花蛇舌草(Hedyotis diffusa Willd)总黄酮的提取工艺及其体外抗氧化活性。以白花蛇舌草总黄酮提取率为评价参数,通过单因素试验、正交试验优化提取工艺;测定了总还原力及Fe2+螯合力、羟自由基和超氧阴离子清除能力研究白花蛇舌草总黄酮的抗氧化活性。结果表明,用95%乙醇与白花蛇舌草粉末按40∶1(m L∶g),在90℃下提取180 min,此时提取率最高,达(2.22±0.01)%。体外抗氧化活性结果显示白花蛇舌草总黄酮具有较强的总还原力,Fe2+螯合率最高为90%,羟自由基和超氧阴离子的清除率分别达79%、77%。白花蛇舌草总黄酮具有显著的体外抗氧化活性,具有进一步开发利用的价值。This study was to optimize the conditions of extraction of total flavonoids from Hedyotis diffusa Willd and detect their antioxidant activities in vitro. Using extraction rate as the evaluation index,single factor experiments and orthogonal test were adopted to optimze the extraction process. Then,four antioxidant parameters of total flavonoids from Hedyotis diffusa Willd,including total reducing power,Fe-（2＋） chelating,hydroxy radical scavenging,and superoxide anion radical scavenging capacities,were measured. The results showed that the optimum conditions of extraction were as following ：ethanol concentration was95%,the ratio of liquid to solid was 40∶1（m L∶g）; extraction time 180 min and temperature,90 ℃. Under these conditions,the extrction ratio was（2.22±0.01）%. The total flavonoids from Hedyotis diffusa Willd had showed high total reducing power,their chelating rate of Fe-（2＋） reached 90%,the radical scavenging activities against hydroxyl radical and superoxide anion were 79%and 77%,respectively. So the antioxidant activities in vitro of flavonoids from Hedyotis diffusa Willd is obvious,which is worth further being developed and utilized.

关 键 词：白花蛇舌草(Hedyotis diffusa Willd) 总黄酮 正交试验 体外抗氧化 

分 类 号：R285.5[医药卫生—中药学]