小蘖碱诱导宫颈癌HeLa细胞系凋亡机制  

Mechanism of berberine-induced apoptosis in cervical cancer HeLa cell line

作　　者：牟凤林[1] 邓晶荣[1] MOU Feng-lin DENG Jing-rong(Chongqing Three Gorges Medical College, Chongqing 404100, China)

机构地区：[1]重庆三峡医药高等专科学校,重庆404000

出　　处：《基础医学与临床》2017年第10期1407-1411,共5页Basic and Clinical Medicine

基　　金：重庆市基础与前沿研究计划(cstc2014jcyj A10049);重庆市社会民生科技创新专项(cstc2016shmszx130028);第二批重庆市高等学校青年骨干教师资助计划(2014)

摘　　要：目的探讨小蘖碱(BR)诱导宫颈癌(He La)细胞系凋亡机制。方法用不同浓度BR和时间处理He La细胞,CCK-8法检测He La细胞增殖效率;流式细胞术(FCM)检测He La细胞周期;Real-time PCR检测细胞内STAT3、CYCLIN B1、CDC2和C-MYC基因的表达量;Wstern blot检测He La细胞内STAT3、CYCLIN B1、CDC2和C-MYC蛋白表达量。结果随着BR浓度增加及作用时间延长,He La细胞的增殖率逐渐降低;BR组中G2/M期He La细胞比值显著高于组(P<0.05);He La细胞经BR处理后STAT3、CYCLIN B1、CDC2和C-MYC基因表达量和蛋白表达量均显著低于对照组(P<0.05)。结论 BR可通过作用于STAT3信号通路来阻滞He La细胞周期,从而诱导细胞凋亡。Objective To study the apoptosis mechanism of He La cells induced by berberine（ BR）. Methods He La cells were treated by different concentrations of BR with different times. CCK-8 assay was used to detect the He La cells proliferation activity. Detecting the expression of STAT3 in control He La cells and BR treated He La cells. He La cell cycles were detected by flow cytometry（ FCM）. The relative expression of STAT3,CYCLIN B1,CDC2 and C-MYC was examined by real-time PCR. The relative expressions of STAT3,CYCLIN B1,CDC2 and C-MYC proteins were examined by Western blot analysis. Results BR can effectively inhibit the proliferation of He La cells in vitro,which exhibits a dose-dependent and time-dependent manner. The FCM showed that the the proportion of BR-treated cells in G2/M phase was higher than control group. Real-time PCR results showed that relative expression of STAT3,CYCLIN B1,CDC2 and C-MYC genes in He La cells treated with BR was lower than the control group. Western blot results showed that relative expression of STAT3,CYCLIN B1,CDC 2 and C-MYC proteins in He La cells treated with BR was lower than the control group. Conclusions BR can induce apoptosis of cervical cancer by targeting STAT3.

关 键 词：小蘖碱 STAT3 细胞周期 凋亡 

分 类 号：R737.33[医药卫生—肿瘤]