Wntless在胃腺癌组织中表达及其意义  

Expression of Wntless in gastric carcinoma and its clinical significance

作　　者：陈昌浩 孙岳军 徐洪明[2] 张建平[1] CHEN Chang-hao SUN Yue-jun XU Hong-ming ZHANG Jian-ping(Dept. of Surgery, the Second Affiliated Hospital of Nanjing Medical University, Nanjing 210011 Dept. of Pathology, the Affiliated Jiangyin Hospital of Southeast University Medical College, Jiangyin 214400, China)

机构地区：[1]南京医科大学第二附属医院普外科,江苏南京210011 [2]东南大学医学院附属江阴医院病理科,江苏江阴214400

出　　处：《基础医学与临床》2017年第10期1440-1443,共4页Basic and Clinical Medicine

摘　　要：目的观察Wnt蛋白分泌相关的基因Wntless在胃癌腺癌细胞和癌旁正常黏膜细胞的表达,分析Wntless表达与胃腺癌病理指标之间相关性和临床意义。方法用免疫组化法检测胃腺癌组织和胃癌旁正常黏膜中Wntless的蛋白表达。结果在104例胃癌标本中,Wntless在胃腺癌组织中表达为:7.7%(8/104)例为0或1+、36.5%(38/104)例为2+、55.8%(58/104)例为3+;在癌旁正常黏膜表达为:71.2%(74/104)例为0或1+,19.2%(20/104)例为2+,9.6%(10/104)例为3+。Wntless在胃腺癌组织中的表达显著高于胃癌旁正常黏膜表达(P<0.001)。Wntless蛋白表达与肿瘤分化呈正相关(P<0.05),与淋巴结转移呈正相关(P<0.05)。结论 Wntless蛋白在胃腺癌中高表达,可能对肿瘤发生和进展等发挥了促进作用,并可能为新的靶向治疗提供分子靶标。Objective To explore the expression of Wnt secret protein Wntless in gastric carcinoma and normal mucosa,and to analyze the clinical significance of Wntless expression. Methods To detecte the expression of Wntless protein in gastric adenocarcinoma and normal gastric mucosa by immunohistochemistry. Results In 104 specimens of gastric cancer,the expression of Wntless in gastric adenocarcinoma was： 7. 7%（ 8/104） cases were 0 or 1 ＋,36. 5%（ 38/104） cases were 2＋,and 55. 8%（ 58/104） cases were 3＋. While in normal mucosa,the expression of Wntless was： 71. 2%（ 74/104） cases were 0 or 1 ＋,19. 2%（ 20/104） cases were 2 ＋,and 9. 6%（ 10/104）cases were 3＋. The expression of Wntless in gastric adenocarcinoma was significantly higher than that in normal gastric mucosa（ P〈0. 001）. The expression of Wntless protein was positively correlated with tumor differentiation（ P〈0. 05）,and was positively correlated with lymph node metastasis（ P〈0. 05）. Conclusions Wntless is highly expressed in gastric carcinomas and may have role in the oncogenesis. Wntless may be used as a new marker for progression and metastasis of gastric carcinoma.

关 键 词：WNT Wntless 胃腺癌 免疫组化 

分 类 号：R735.2[医药卫生—肿瘤]