转内切葡聚糖酶植物乳杆菌对断奶獭兔生产性能与免疫性能的影响  

Effect of transformed lactobacillus with endoglucanases gene on growth and immune performance of rex rabbits

作　　者：胡志刚[1] 任战军[1] 宋冰[1] 李倩[1] 宫瑞光[1] HU Zhi-gang REN Zhan-jun SONG Bing LI Qian GONG Rui-guang(College of Animal Science and Technology ,Northwest A＆F University ,Yangling ,Shaanli 712100 ,China)

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《中国兽医学报》2017年第10期2020-2026,共7页Chinese Journal of Veterinary Science

基　　金：陕西省农业攻关基金资助项目(2010K01-16)

摘　　要：本试验通过饮水添加转内切葡聚糖酶基因植物乳杆菌(L.plantarum pLEM-cel),探究L.plantarum pLEM-cel对30日龄断奶獭兔生产性能和免疫性能的影响。将30日龄健康獭兔随机分为3组(每组20只,每只为1个重复)。对照组饲喂基础日粮,试验组在饲喂基础日粮的基础上分别饮水添加L.plantarum pLEM-cel和植物乳杆菌(L.plantarum)。试验期31d。结果显示,饮水添加L.plantarum pLEM-cel可提高平均日增重,显著降低断奶獭兔的料重比(P<0.05);显著提高断奶獭兔的全净膛率和胸腺指数、十二指肠绒毛高度和绒毛高度/隐窝深度(v/c)以及回肠绒毛高度(P<0.05)。添加L.plantarum pLEM-cel可显著提高断奶獭兔的血清IgG和肠黏膜SIgA含量、小肠黏膜IFN-γ及IL-2基因的相对表达量。结果表明,饮水添加L.plantarum pLEM-cel,可以提高断奶獭兔的生产性能,改善机体的免疫水平。This experiment aimed to explore the effect of endoglucanase-producing laetobacillus on growth and immune performance of 30-day-old weaned rex rabbit. Sixty weaned young rabbits at 30-day-old with similar weight were randomly divided into 3 groups with 20 replicates per group. The control group was fed a basal diet only, and the test groups were fed the basal diet with L. plantarum pLEM-cel and Lactobacillus plantarum, respectively. The trial lasted 31d. The results showed that compared with contral group,the F/G significantly reduced（P^0.05） in weaning rex rabbits with L. plantarum pLEM-cel, the eviscerated rate and thymus index significantly improved （P〈0. 05） in weaning rex rabbits with L. plantarum pLEM-cel. Supplementing L. plantarurn pLEM-cel improved the villus height and V/C of duodenal and villus height of ileal significantly （P〈0.05） and the contents of serum IgG and mucosa SIgA and the relative expression of IFN-γ and IL-2 in intestine of weaning rex Rabbits with L. plantarum pLEM-cel were significantly improved（P〈0.05）, compared with contral group,These results suggested that the L. plantarurn pLEM-cel is an ideal additive to improve the growth performance and immunity of rex rabbit.

关 键 词：转内切葡聚糖酶乳酸杆菌 植物乳杆菌 獭兔 生产性能 免疫性能 

分 类 号：S829.1[农业科学—畜牧学]