麻椒总黄酮的纯化工艺及其抗氧化性研究  

Study on Purification Technology and Antioxidant Activity of Total Flavonoids from Pepper

作　　者：王晓林[1] 金龙哲[2] 钟方丽[1] 秦杰 WANG Xiao-lin JIN Long-zhe ZHONG Fang-li QIN Jie(School of Chemistry and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China Yanbian Korean Autonomous Prefecture Academy of Agricultural Sciences,Yanji 133001, China)

机构地区：[1]吉林化工学院化学与制药工程学院,吉林吉林132022 [2]延边朝鲜族自治州农业科学院,吉林延吉133001

出　　处：《中国调味品》2017年第10期33-40,共8页China Condiment

摘　　要：对麻椒总黄酮的纯化工艺条件及其体外抗氧化活性进行了研究。以总黄酮的比吸附量、吸附率及比解吸量、解吸率为考察指标,采用单因素实验对麻椒总黄酮的纯化工艺进行考察;采用分光光度法探索了麻椒总黄酮对羟基自由基、超氧阴离子自由基的清除活性。结果表明:LSA-21型大孔吸附树脂对麻椒总黄酮纯化的较佳工艺为:控制麻椒提取液总黄酮质量浓度0.96mg/mL左右,然后用石油醚脱脂,脱脂后麻椒提取液的上样体积与树脂质量之比(mL/mg)为6∶1,吸附流速控制在1.5mL/min,解吸液pH 8.0的90%乙醇水溶液,解吸流速控制在1.5mL/min,解吸液体积与树脂质量之比(mL/mg)为10∶1。在此纯化条件下,LSA-21型树脂对麻椒总黄酮的比吸附量平均为5.248mg/g、吸附率平均为90.79%,比解吸量平均为5.032mg/g、解吸率平均为95.88%,干浸膏中总黄酮含量由12.12%提高到36.65%。体外抗氧化活性的研究表明:麻椒总黄酮对羟基自由基、超氧阴离子自由基的清除能力较强,均呈现出量效关系,其清除羟基自由基、超氧阴离子自由基的IC50分别为0.1946,0.1336mg/mL。麻椒总黄酮的抗氧化活性与其浓度呈正相关,具有质量浓度依赖性的体外抗氧化活性,其清除自由基能力强于维生素C,表明麻椒总黄酮具有较强的体外抗氧化活性。研究结果为麻椒的进一步开发利用提供了依据。The process for purifying total flavonoids from pepper and the antioxidant activity of total flavonoids are studied. The purification process of TFs is optimized by single factor and orthogonal tests using adsorption capacity, adsorption ratio, desorption capacity, desorption ratio as indexes for investigation. The scavenging activity of total flavonoids from pepper on hydroxyl radical and superoxide anion free radical is studied by spectrophotometry. The results show that the optimum purification conditions of TFs in pepper are as follows： keep the total flavonoids concentration at about 0. 96 mg/mL,and then degrease with petroleum ether, the ratio of sample volume of defatted pepper extract with resin mass （mL/mg） is 6 ： 1 ,the adsorption velocity is 1.5 mL/min, the eluate is 90% （volume fraction） ethanol solution（pH 8. 0）, the desorption velocity is 1.5 mL/min, the ratio of the desorption volume with resin mass （mL/mg） is 10 ： 1. Under the conditions of purification, the average adsorption capacity of LSA-21 resin to total flavonoids is 5. 248 mg/g, the average adsorption ratio is 90.79 % ,the average desorption capacity of LSA-21 resin to total flavonoids is 5. 032 mg/g, the average desorption rate is 95.88 %, the purity of TFs in pepper extract could be changed from 12.12% to 36.65%. The scavenging ability of total flavonoids from pepper to hydroxyl radical and superoxide anion radical is stronger, both shows dose-response relationship, scavenging hydroxyl radical, superoxide anion radical IC50 values are 0. 1946, 0. 1336 mg/mL. The antioxidant activity of total flavonoids is positively correlated with its concentration, in vitro antioxidant activity with mass concentration dependent, its ability to scavenge free radicals is stronger than vitamin C. The results show that the total flavonoids have strong antioxidant activity in vitro. The results have provided basis for further development and utilization of pepper.

关 键 词：麻椒 总黄酮 大孔吸附树脂 抗氧化 

分 类 号：TS201.1[轻工技术与工程—食品科学]