慢病毒介导lncRNA-AK058003过表达对肝癌裸鼠移植瘤生长的影响  

Effects of lncRNA-AK058003 Overexpression on the Growth of Human Hepatocellular Carcinoma in Nude Mice

作　　者：周宇杰[1] 徐细明[1] 梁慧玲[1] Zhou Yujie Xu Ximing Liang Huiling(Department of Oncology, Renmin Hospital of Wuhan University, Hubei 430060, Chin)

机构地区：[1]武汉大学人民医院肿瘤科,430060

出　　处：《医学研究杂志》2017年第9期46-50,共5页Journal of Medical Research

基　　金：湖北省自然科学基金资助项目(2012FKC14301)

摘　　要：目的探究上调SK-Hep1肝癌细胞中lnc RNA-AK058003的表达对肝癌裸鼠移植瘤生长的影响。方法采用慢病毒转染的方法将lnc RNA-AK058003的重组质粒和空质粒分别转入SK-Hep1肝癌细胞株中,经新霉素筛选出lnc RNAAK058003稳定过表达的细胞株,用定量PCR的方法检测lnc RNA-AK058003在SK-Hep1肝癌细胞中的过表达,并且将稳定过表达lnc RNA-AK058003的SK-Hep1肝癌细胞和含有空质粒的SK-Hep1肝癌细胞分别注射入裸鼠腋下皮下,定期测量裸鼠移植瘤的长径和短径,从而计算出移植瘤的体积,饲养35天后测量移植瘤的重量。结果构建稳定过表达lnc RNA-AK058003的肝癌细胞株,裸鼠皮下荷瘤实验结果显示,过表达组裸鼠移植瘤的体积(308.4±439.4mm^3)、重量(0.464±0.518g)和生长速度均低于空质粒组(1410.0±973.0mm^3,1.363±0.856g,P<0.05)。结论 lnc RNA-AK058003对肝癌裸鼠移植瘤的增殖具有抑制作用。Objective To investigate up - regulation of lncRNA - AK058003 expression in SK - Hepl hepatocellular carcinoma ceils and its effect on the growth of human hepatocellular carcinoma xenograft tumor in nude mice. Methods The recombinant plasmid of lncRNA - AK058003 and empty plasmid were transferred into SK - Hepl human hepat0cellular carcinoma cell line by the method of slow virus transfection. The overexpression of lncRNA - AK058003 cell line was screened by neomycin. Overexpression of lncRNA - AK058003 in SK - Hepl hepatocarcinoma cells was detected by quantitative PCR. SK - Hepl hepatocellular carcinoma cells stably over- expressing lncRNA - AK058003 and SK - Hepl hepatocellular carcinoma cells containing empty plasmids were injected subcutaneously in- to armpit of nude mice. The long and short diameters of the transplanted tumor were measured regularly to calculate the volume of the xen- ograft tumor. After 35 days of feeding we measure the weight of the xenograft tumor. Results The recombinant plasmid of lncRNA - AK058003 was successfully constructed and stably over expressed in hepatocellular carcinoma cells. The experimental results of subcuta- neous tumor bearing nude mice showed that the volume （308.4 ± 439.4mm3） , weight （0. 464 ± 0.518g） and growth rate of the xenograft tumor in the over expression group were lower than that of the empty plasmid group（ 1410.0 ± 973.0mm3 , 1. 363 ± 0. 856g,P 〈 0.05 ）. Conclusion LncRNA - AK058003 has inhibitory effect on the proliferation of human hepatocellular carcinoma xenograft tumor in nude mice.

关 键 词：lncRNA-AK058003 肝癌 裸鼠移植瘤 

分 类 号：R735.7[医药卫生—肿瘤]