USPIO标记脐带间充质干细胞靶向小鼠肺癌MR、病理对照研究  

MRI and pathological study of targeted mouse lung cancer with USPIO-labeled umbilical cord mesenchymal stem cells

作　　者：王雪芹[1] 张永峰[2] 张淑娴[1] 王锡臻[1] 徐芹艳[1] 

机构地区：[1]潍坊医学院附属医院影像中心,山东潍坊261031 [2]潍坊医学院附属医院儿科,山东潍坊261031

出　　处：《实用放射学杂志》2017年第10期1619-1622,共4页Journal of Practical Radiology

基　　金：山东省医药卫生科技发展项目基金（2013WS0294）.

摘　　要：目的 将USPIO-PLL标记人脐带间充质干细胞（MSCs）移植入肺癌LLC种植瘤的小鼠，利用3.0T MR对MSCs活体示踪；〖JP〗运用〖JP3〗免疫组化SABC法，探讨MSCs与血管内皮细胞生长因子（VEGF）表达及肿瘤血管的关系；综合分析移植MSCs对肺癌的影响。方法 培养人脐带MSCs；以多聚赖氨酸（PLL）为转染剂，用USPIO对MSCs-PLL进行磁性标记；将磁标记的MSCs通过鼠尾静脉植入LLC种植瘤小鼠体内，分别于移植前、移植后1 d、10 d行MRI观察；组织块行免疫组化。结果 （1）移植后1 d MRI可监测到MSCs到达肿瘤区，10 d到达肿瘤区MSCs增多，MRI信号降低，差异有统计学意义（P〈0.05）；移植1 d、10 d抑瘤率均为正值；（2）移植后10 d，MSCs移植组VEGF表达阳性率为86.67%，MVD值为44.22±12.36；NS组VEGF表达阳性率为26.67%，MVD值为20.29±8.47；VEGF阳性表达及微血管密度（MVD）值组差别均有统计学意义（P〈0.05）。结论 3.0T MR可以有效进行干细胞移植后的活体示踪；干细胞对肺癌具有双向作用：既可定向趋化分化抑制肿瘤生长，也可一定程度增强VEGF表达及微血管形成。Objective To trace human umbilical cord mesenchymal stem cells （MSCs） labelled by USPIO-PLL which were transplanted into mouse subcutaneous xenotransplanted lung cancer by using 3.0T MR, to investigate the relationship between MSCs and VEGF expression and tumor angiogenesis by using SABC immunohistochemical method and to comprehensively analyze the effect of MSCs transplantation on lung cancer.Methods Cultured MSCs and poly lysine （PLL） was as a transfection agent which was magnetically labeled by USPIO and implanted into mice with subcutaneous xenotransplanted lung cancer through the tail vein.MRI was performed at pre-transplantation, 1 d and 10 d after transplantation and the tissues were performed by immunohistochemistry respectively. Results （1） MSCs could reach the tumor area at the first day after the transplantation and be monitored by MRI. MSCs increased at the 10th day. MRI signal intensity was reducedand the difference was statistically significant （P〈0.05）.The inhibitory rate of the 1st day and 10th day was positive; （2） At the 10th day after the transplantation, the rate of the VEGF positive expression in MSCs group was 86.67%, the value of MVD was 44.22 ± 12.36, and the rate of the VEGF positive expression in NS group was 26.67%, the value of MVD was 20.29±8.47 （P〈0.05）.Conclusion Tracing stem cell transplantation in vivo can be proceeded effectively by using 3.0T MR. Stem cell has bidirectional effect on lung cancer which inhibites the tumor growth by directional chemotaxis and differentiation, and also enhances expression of VEGF and angiogenesis at a certain extent.

关 键 词：间充质干细胞 肺癌 USPIO 血管内皮细胞生长因子 微血管密度 磁共振成像 

分 类 号：R734.2[医药卫生—肿瘤] R445.2[医药卫生—临床医学]