双氢青蒿素对UVB照射小鼠皮肤损伤的光保护作用  

Protetive Effect of Dihydroartemisinin on Chronic Photic Injury Induced by UVB in Mouse Skin

作　　者：惠海英[1] 吴娜[1] 孙晶莹[2] 宫艳艳[3] 张美芳[1] 肖生祥[4] 

机构地区：[1]陕西省人民医院皮肤科,陕西西安710068 [2]陕西省人民医院中心实验室,陕西西安710068 [3]陕西省人民医院检验科,陕西西安710068 [4]西安交通大学第二附属医院皮肤科,陕西西安710004

出　　处：《中国皮肤性病学杂志》2017年第10期1061-1064,1084,共5页The Chinese Journal of Dermatovenereology

摘　　要：目的研究双氢青蒿素对中波紫外线照射后小鼠皮肤组织白介素(interleukin,IL)-1,IL-6及半胱氨酸天冬氨酸蛋白酶(cysteinylaspartic speahc proteinase)-3,Caspase-8表达的影响,初步探讨青蒿素光保护作用的可能机制。方法 40只BALB/C小鼠随机分为4组,即对照组(C),NB-UVB照射组(UVB),双氢青蒿素组(DHA),双氢青蒿素+NB-UVB组(DHA+UVB)。C组给予生理盐水灌胃,UVB组给予生理盐水灌胃后NB-UVB照射,DHA组给予双氢青蒿素灌胃,DHA+UVB组给予双氢青蒿素灌胃后进行NB-UVB照射。各组灌胃100mg/(kg·d),持续2周。UVB照射剂量为1 J/(cm^2·d),共2周,累积剂量14 J/cm^2,14天后取小鼠背部皮肤组织,采用酶联免疫吸附试验检测各组小鼠皮肤组织IL-1,IL-6水平,RT-PCR,WT-blot检测各组小鼠皮肤组织Caspase-3,Caspase-8变化情况。结果与C组相比,UVB组皮肤组织中IL-1,IL-6明显增加,Caspase-3,Caspase-8 mRNA表达及其蛋白水平均增加,差异均有统计学意义(均P<0.05),DHA组小鼠皮肤组织IL-1,IL-6水平没有明显变化;Caspase-3,Caspase-8表达降低,但差异均无统计学意义(均P>0.05)。DHA+UVB组与UVB组相比,IL-1,IL-6的浓度增加明显减少,Caspase-3,Caspase-8 mRNA表达及蛋白水平降低,差异均有统计学意义(均P<0.05)。结论双氢青蒿素对紫外线所致小鼠光损伤的炎症因子IL-1,IL-6及凋亡蛋白Caspase-3,Caspase-8的产生有一定的抑制作用。提示系统性应用双氢青蒿素对UVB致小鼠光损伤有保护作用。Objective To study the protective effect of dihydroartemisinin on chronic skin photodamage, and how it influcnces the expression of IL-1,IL-6,Caspase-3,Caspase-8 on mouse skin induced by UVB.Methods Forty BALB/C mouse were randomized into four groups, namely,comprising control group（C）,ultraviolet exposed group（UVB）,dihydroartemisinin injection-treated group（DHA）and dihydroartemisinin injection-treated and ultraviolet exposed group（DHA＋UVB）.Mouse of the normal control group were injected with normal sodium; Mouse of UVB group were irradiated with NB-UVB radiation（after the injection of normal sodium）; DHA group were injected with dihydroartemisinin and DHA＋UVB group were treated with dihydroartemisinin injection and ultraviolet exposure.A dose of 100 mg/kg and a dose of 1 J/cm2 was given every day for 2 weeks.After NB-UVB radiation,the skin specimens were obtained.ELISA method was used to detect the Caspase-3 and Caspase-8 protein expression in these specimens.Results Compared with the c group,there was a significant increase in the expression of IL-1,IL-6,Caspease-3,Caspease-8 in UVB and DHA＋UVB group（all P〈0.05）,but compared with the UVB group,IL-1,IL-6,Caspease-3,Caspase-8 expression levels in the DHA＋UVB group decline（ all P〈0.05）.Conclusion Our results suggest that dihydroartemisinin can protect against the damage induced by UVB in mouse skin,and IL-1,IL-6,Caspease-3,Caspase-8 maybe play a role.

关 键 词：双氢青蒿素 白介素-1 白介素-6 半胱氨酸天冬氨酸蛋白酶-3 半胱氨酸天冬氨酸蛋白酶-8 中波紫外线 

分 类 号：R363.122[医药卫生—病理学] R758.1[医药卫生—基础医学]