FBXO31基因促进顺铂诱导的肝癌细胞凋亡  

FBXO31 promotes cisplatin-induced apoptosis in hepatocellular carcinoma

作　　者：何惠娟 黄海丽 王镇南 杨拉维 陈婷 吴平 

机构地区：[1]广东医科大学附属医院临床医学研究中心,广东湛江524001 [2]广东医科大学附属医院肿瘤科,广东湛江524001

出　　处：《广东医科大学学报》2017年第4期350-352,共3页Journal of Guangdong Medical University

基　　金：国家自然科学基金(No.81600484);广东医科大学附属医院博士启动项目(No.BK201202);广东医科大学科研基金立项资助项目(No.M2016009)

摘　　要：目的探讨FBXO31基因对顺铂诱导肝癌细胞凋亡的影响。方法通过脂质体转染法将FBXO31基因表达质粒转染至肝癌HepG2细胞,再用2 mg/L顺铂处理,分别用MTT、流式细胞术、免疫印迹检测细胞活性、凋亡、FBXO31蛋白表达。结果与pcDNA组比较,FBXO31组细胞活力显著降低,细胞凋亡明显增多,FBXO31蛋白表达显著升高(P<0.01或0.05)。结论上调FBXO31基因表达促进顺铂诱导的肝癌细胞凋亡。Objective To investigate the effect of FBXO31 on cisplatin-induced apoptosis in hepatocellular carcinoma. Methods FBXO31 expression plasmids were transfected into HepG2 cells using lipofection. After treated with 2 mg/L cisplatin, the viability, apoptosis, and FBXO31 protein expression of HepG2 cells were determined by MTT, flow cytometry, and Western blot, respectively. Results Compared with pcDNA group, cell viability was decreased, while apoptosis and FBXO31 expression were increased in FBXO31 group （P〈0.01 or 0.05）. Conclusion Overexpression of FBXO31 gene promotes cisplatin-induced apoptosis in hepatocellular carcinoma.

关 键 词：肝癌 FBXO31 顺铂 细胞凋亡 

分 类 号：R730.52[医药卫生—肿瘤]